@article {Abdelbari2021,
	title = {Implementing spanlastics for improving the ocular delivery of clotrimazole: In vitro characterization, ex vivo permeability, microbiological assessment and in vivo safety study},
	journal = {International Journal of Nanomedicine},
	volume = {16},
	year = {2021},
	note = {n/a},
	abstract = {<p>{Purpose: The aim of this study was to encapsulate clotrimazole (CLT), an antifungal drug with poor water solubility characteristics, into spanlastics (SPs) to provide a controlled ocular delivery of the drug. Methods: Span 60 was used in the formulation of SPs with Tween 80, Pluronic F127, or Kolliphor RH40 as an edge activator (EA). The presence of EA offers more elasticity to the membrane of the vesicles which is expected to increase the corneal permeation of CLT. SPs were prepared using ethanol injection method applying 32 complete factorial design to study the effect of formulation variables (ratio of Span 60: EA (w/w) and type of EA) on SPs characteristics (encapsulation efficiency percent (EE\%), average vesicle size (VS), polydis-persity index (PDI) and zeta potential (ZP)). Design-Expert software was used to determine the optimum formulation for further investigations. Results: The optimum formulation determined was S1, which contains 20 mg of Tween 80 used as an EA and 80 mg of Span 60. S1 exhibited EE\% = 66.54 {\textpm} 7.57\%</p>
},
	issn = {11782013},
	doi = {10.2147/IJN.S319348},
	author = {Manar Adel Abdelbari and Shereen Sameh El-Mancy and Elshafeey, Ahmed Hassen and Aly Ahmed Abdelbary}
}
