@article {MURIN202389,
	title = {Porcine oocytes matured in a chemically defined medium are transcriptionally active},
	journal = {Theriogenology},
	volume = {203},
	year = {2023},
	note = {n/a},
	pages = {89-98},
	abstract = {<p>The statement that fully-grown porcine oocytes (oocytes from follicles with diameter from 3 to 6~mm) are transcriptionally quiescent is not as strongly supported as it was before. Currently, we know that there is a difference between the transcription profile of germinal vesicle (GV) and metaphase II (MII) oocytes. The goal of our study was to compare the transcription profile of GV, germinal vesicle breakdown (GVBD), metaphase I (MI), and MII oocytes matured in the chemically defined medium FLI. Oocytes were sequenced, and the results were subsequently validated using quantitative reverse transcription polymerase chain reaction (RT-qPCR). We detected multiple differentially transcribed mRNAs, of which many were upregulated. Among them we found mRNAs necessary for protein production, mitochondrial functions and cytoplasmic maturation. Collectively, these data support the hypothesis that transcription activity in fully-grown porcine oocytes is necessary for key processes during their successful maturation in~vitro in a chemically defined maturation medium.</p>
},
	keywords = {Chemically defined medium, maturation, Porcine oocyte, Transcriptome},
	issn = {0093-691X},
	doi = {https://doi.org/10.1016/j.theriogenology.2023.03.019},
	url = {https://www.sciencedirect.com/science/article/pii/S0093691X23001097},
	author = {Murin, Matej and Nemcova, Lucie and Bartkova, Alexandra and Gad, Ahmed and Lucas-Hahn, Andrea and Strejcek, Frantisek and Prochazka, Radek and Laurincik, Jozef}
}
