@article {ani11030794,
	title = {Adaptive and Biological Responses of Buffalo Granulosa Cells Exposed to Heat Stress Under In Vitro Condition},
	journal = {Animals},
	volume = {11},
	number = {3},
	year = {2021},
	note = {n/a},
	abstract = {<p>The steroidogenesis capacity and adaptive response of follicular granulosa cells (GCs) to heat stress were assessed together with the underlying regulating molecular mechanisms in Egyptian buffalo. In vitro cultured GCs were exposed to heat stress treatments at 39.5, 40.5, or 41.5 {\textdegree}C for the final 24 h of the culture period (7 days), while the control group was kept under normal conditions (37 {\textdegree}C). Comparable viability was observed between the control and heat-treated GCs at 39.5 and 40.5 {\textdegree}C. A higher release of E2, P4 and IGF-1 was observed in the 40.5 {\textdegree}C group compared with the 39.5 or 41.5 {\textdegree}C groups. The total antioxidant capacity was higher in response to heat stress at 39.5 {\textdegree}C. At 40.5 {\textdegree}C, a significant upregulation pattern was found in the expression of the stress resistance transcripts (SOD2 and NFE2L2) and of CPT2. The relative abundance of ATP5F1A was significantly downregulated for all heat-treated groups compared to the control, while TNFα was downregulated in GCs at 39.5 {\textdegree}C. Expression analyses of stress-related miRNAs (miR-1246, miR-181a and miR-27b) exhibited a significant downregulation in the 40.5 {\textdegree}C group compared to the control, whereas miR-708 was upregulated in the 39.5 and 40.5 {\textdegree}C groups. In conclusion, buffalo GCs exhibited different adaptive responses, to the different heat stress conditions. The integration mechanism between the molecular and secretory actions of the GCs cultured at 40.5 {\textdegree}C might provide possible insights into the biological mechanism through which buffalo GCs react to heat stress.</p>
},
	issn = {2076-2615},
	doi = {10.3390/ani11030794},
	url = {https://www.mdpi.com/2076-2615/11/3/794},
	author = {Faheem, Marwa S. S. and Ghanem, Nasser and Gad, Ahmed and Proch{\'a}zka, Radek and Dessouki, Sherif M. M.}
}
