@article {196567,
	title = {Plasma extracellular vesicle miRNAs as potential biomarkers of superstimulatory response in cattle},
	journal = {Scientific Reports},
	volume = {10},
	number = {1},
	year = {2020},
	note = {<p>n/a</p>
},
	month = {dec},
	pages = {1{\textendash}16},
	publisher = {Nature Publishing Group UK},
	abstract = {<p>The ability to predict superstimulatory response would be a beneficial tool in assisted reproduction. Using small RNAseq technology, we profiled extracellular vesicle microRNA (EV-miRNA) abundance in the blood plasma of heifers exhibiting variable responses to superstimulation. Estrous synchronized crossbred beef heifers (n = 25) were superstimulated and blood samples were collected from each heifer on Day 7 of consecutive unstimulated (U) and superstimulated (S) cycles. A subset of high (H) and low (L) responders was selected depending on their response to superstimulation and EV-miRNA profiles were analysed at both time-points in each heifer. Approximately 200 known miRNAs were detected in each sample with 144 commonly detected in all samples. A total of 12 and 14 miRNAs were dysregulated in UH vs. UL and in SH vs. SL heifers, respectively. Interestingly, miR-206 and miR-6517 exhibited the same differential expression pattern in H compared to L heifers both before and after superstimulation. Pathway analysis indicated that circadian rhythm and signaling pathways were among the top pathways enriched with genes targeted by dysregulated miRNAs in H vs. L responding heifers. In conclusion, heifers with divergent ovarian responses exhibited differential expression of plasma EV-miRNAs which may be used as a potential biomarker to predict superstimulation response.</p>
},
	isbn = {0123456789},
	issn = {2045-2322},
	doi = {10.1038/s41598-020-76152-9},
	url = {https://doi.org/10.1038/s41598-020-76152-9 http://www.nature.com/articles/s41598-020-76152-9},
	author = {Gad, Ahmed and S{\'a}nchez, Jos{\'e} Mar{\'\i}a and Browne, John A and Nemcova, Lucie and Laurincik, Jozef and Prochazka, Radek and Lonergan, Pat}
}
