Detection of Dead stained microscopic cells based on Color Intensity and Contrast

TarasKotyk, N. D., A. S. Ashour, A. D. C. Victoria, T. Gaber, A. E. Hassanien, and V. Snasel, "Detection of Dead stained microscopic cells based on Color Intensity and Contrast", The 1st International Conference on Advanced Intelligent System and Informatics (AISI2015), 2015, , Beni Suef, Egypt, November 28-30, , 2015.

Date Presented:

November 28-30,


Apoptosis is an imperative constituent of various processes including proper progression and functioning of the immune system, embryonic development as well as chemical-induced cell death. Improper apoptosis is a reason in numerous human/animal’s conditions involving ischemic damage, neurodegenerative diseases, autoimmune disorders and various types of cancer. An outstanding feature of neurodegenerative diseases is the loss of specific neuronal populations. Thus, the detection of the dead cells is a necessity. This paper proposes a novel algorithm to achieve the dead cells detection based on color intensity and contrast changes and aims for fully automatic apoptosis detection based on image analysis method. A stained cultures images using Caspase stain of albino rats hippocampus specimens using light microscope (total 21 images) were used to evaluate the system performance. The results proved that the proposed system is efficient as it achieved high accuracy (98.89 ± 0.76 %) and specificity (99.36 ± 0.63 %) and good mean sensitivity level of (72.34 ± 19.85 %).



Related External Link