Detection of Dead stained microscopic cells based on Color Intensity and Contrast

Gaber, T., T. Kotyk, N. Dey, A. D. C. V. Amira Ashour, A. E. Hassanienan, and V. Snasel, "Detection of Dead stained microscopic cells based on Color Intensity and Contrast", the 1st International Conference on Advanced Intelligent Systems and Informatics (AISI’15) , Springer. , Beni Suef University, Beni Suef, Egypt, Nov. 28-30, 2015.

Date Presented:

Nov. 28-30, 2015


Apoptosis is an imperative constituent of various processes including proper
progression and functioning of the immune system, embryonic development as well
as chemical-induced cell death. Improper apoptosis is a reason in numerous human/
animal’s conditions involving ischemic damage, neurodegenerative diseases,
autoimmune disorders and various types of cancer. An outstanding feature of
neurodegenerative diseases is the loss of specific neuronal populations. Thus, the
detection of the dead cells is a necessity. This paper proposes a novel algorithm to
achieve the dead cells detection based on color intensity and contrast changes and
aims for fully automatic apoptosis detection based on image analysis method. A
stained cultures images using Caspase stain of albino rats hippocampus specimens
using light microscope (total 21 images) were used to evaluate the system
performance. The results proved that the proposed system is efficient as it achieved
high accuracy (98.89 ± 0.76 %) and specificity (99.36 ± 0.63 %) and good mean
sensitivity level of (72.34 ± 19.85 %).