Mohamed Zaki

IF: 2.602

This study was designed to evaluate the potential chemopreventive activities of Ginkg biloba extract (EGb) and Silybum marianum extract (silymarin) against hepatocarcinogenesis induced by N-nitrosodiethylamine (ND EA) in rats. Rats were divided into 6 groups. Group 1 served as normal control rats. Group 2 animals were intragastrically administrated N DEA a t a dose of 10 mg/k g five times a week for 12 weeks to induce hepatocellular carcinoma (HCC). Groups 3 and 4 animals were pretreated with silymarin and E Gb respectively. Groups 5 and 6 animals were post treated with silymarin and EGb respectively. The investigated parameters in serum alanine aminotransferase (ALT), aspartate a minotrasferase (AST), gamma glutamyltransferase (GGT) and vascular endothelial growth factor (VEGF). The investigated parameters in liver tissue are malondialdehyde (MDA), glutathione (GS H ), superoxide dismutase ( SOD), glutathione peroxidase (GPx), glutathione reductase (GR) and comet assay parameters.

Chitosan is a polysaccharide that has gained interest in recent to encapsulate active compounds due to its non-toxicity, biodegradability, biocompatibility and immunity system induction. The chitosan nanoparticles was prepared by adding vitamin C to tripolyphosphate (TPP) solution and then blending it stepwise to the chitosan solution under stirring. Nanoparticle characterizations were analyzed by scanning electron microscopy (SEM) and Zetasizer. The release profile of vitamin C into the gastrointestinal and serum of rainbow trout (Oncorhynchus mykiss) was portrayed and its effect on the immunity system of the fish was determined as well. The results showed that the chitosan nanoparticle had spherical shape; positive charge with particle size of 185 nm and in vivo controlled release until 48 h. Lysozyme and complement contents in the fish serum increased as long as they were provoked by the nanoparticle. This nanoparticles is a promising technique to boost immunity system of fish.

The aim of this study was to determine the epidemiology of chlamydiosis in free ranging asymptomatic and diarrhoeic sheep and goats. Fecal swabs were examined for the presence of chlamydiae by culture in Vero cells, chick embryo, Gimenez staining, direct fluorescein-conjugated monoclonal antibody staining and immunoperoxidase. Chlamydial DNA was then used to identify specific DNA by omp2 gene using the PCR-RFLP. The asymptomatic goats were 50% positive for the presence of the omp2 gene of the family Chlamydiaceae which were totally Cp. psittaci (100%). The percentage of the family Chlamydiaceae in diseased goats was 16.2% which were also identified to be positive for Cp. psittaci (100%). The asymptomatic sheep revealed that 6.7% were positive for the presence of the omp2 gene of the family Chlamydiaceae and to be 100% positive for Cp. psittaci. On the other hand, 42.9% of the samples that were collected from the diseased sheep were positive for the family Chlamydiaceae of which 25.7% were Cp. psittaci and 4.8% Cp. pecorum.

Purpose: The aim of this study was to evaluate and compare the effect of changing either the amount of mandibular protrusion or the vertical jaw separation on apnea/hypopnea index (AHI) and snoring index (SI) In patients suffering from obstructive sleep apnea (OSA).

Purpose: The aim of this study was to evaluate and compare the effect of changing either the amount of mandibular protrusion or the vertical jaw separation on apnea/hypopnea index (AHI) and snoring index (SI) In patients suffering from obstructive sleep apnea (OSA).

Tamoxifen citrate (TC) is a selective estrogen receptor modifier (SERM) that is usually used in the treatment of breast cancer which is associated with high levels of cyclooxygenase enzyme. Some new non-steroidal anti-inflammatory drugs (NSAIDs) are effective in preventing estrogen receptor-positive tumors, which currently can be prevented and treated with drugs such as tamoxifen citrate. In this work, a high performance liquid chromatographic method is described for the determination of the (TC) in the presence of some usually prescribed NSAIDs, namely, ibuprofen or paracetamol. The analysis is carried out on Zorbax ODS C18 column using mobile phase consisting of methanol: 1 % triethylamine (89: 11, by volumes) at pH = 4 (adjusted using o-phosphoric acid) and flow rate of 1 ml/min. The method has been validated according to USP guidelines and the system suitability parameters have been calculated. The method is successfully applied for the determination of the drugs in pure powdered forms, pharmaceutical dosage formulations and in spiked plasma samples.

Tamoxifen citrate (TC) is a selective estrogen receptor modifier (SERM) that is usually used in the treatment of breast cancer which is associated with high levels of cyclooxygenase enzyme. Some new non-steroidal anti-inflammatory drugs (NSAIDs) are effective in preventing estrogen receptor-positive tumors, which currently can be prevented and treated with drugs such as tamoxifen citrate. In this work, a high performance liquid chromatographic method is described for the determination of the (TC) in the presence of some usually prescribed NSAIDs, namely, ibuprofen or paracetamol. The analysis is carried out on Zorbax ODS C18 column using mobile phase consisting of methanol: 1 % triethylamine (89: 11, by volumes) at pH = 4 (adjusted using o-phosphoric acid) and flow rate of 1 ml/min.
The method has been validated according to USP guidelines and the system suitability parameters have been calculated. The method is successfully applied for the determination of the drugs in pure powdered forms, pharmaceutical dosage formulations and in spiked plasma samples.

The influences of medicinal plants Juniperus Phoenicea (Araar), Hyphaene thebaica (Doum), Anastatica hierochuntica (Kafta) and Cleome droserifolia (Sammo) as antidiabetic agents were investigated using female pregnant albino rats. Female rats were injected with 60 mg/kg b.w. alloxan to induce diabetes. Diabetic rats treated orally with the methanol extracts of tested plants till the 19 day of gestation.

Background: Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm associated with the BCR-ABL fusion gene (the Philadelphia translocation, Ph). In philadelphia-positive CML, abnormalities of chromosome 20 were also detected, which were associated, in the majority of cases, with clonal evolution and disease progression to accelerated phase or blastic crisis. Aim of the Work: Was to study the prevalence of chromosome 20q aberrations in an Egyptian cohort of Ph positive-CML and to evaluate the correlation of these genetic defects with the clinical presentation, laboratory data, disease progression and evolution into accelerated phase or blastic crisis, treatment outcome as well as overall survival. Patients and Methods: This study included 30 Ph positive-CML patients as well as 15 age and sex matched normal healthy subjects as a control group. All patients were subjected to Fluorescence In Situ Hybridization (FISH) technique for detection of chromosome 20q aberrations. Results: Our study revealed that 14/30 Ph positive-CML patients (47%) had aberrations of chromosome 20q. Of these 4/16 patients (25%) were in the chronic phase and 10/14 patients (72%) were in the evolution phase. Nine of the thirty patients (30%) had del 20q12; 3/16 patients (19%) in the chronic phase and 6/14 patients (43%) in the evolution phase. Trisomy 20q was found in 5/30 patients (17%); 1/16 patients (6%) in the chronic phase and 4/14 patients (29%) in the evolution phase. Del 20q12 and trisomy 20q cytogenetic abnormalities were associated with significant increased risk of the evolution phase in CML with OR 2.826 & 2.915 and 95%CI 1.845-9.473 & 1.852-9.879 respectively. Ph-positive CML patients with del 20q12 or trisomy 20q had a significant increased number of chemotherapy cycles, shorter total free survival and overall survival (p<0.05) and a highly significant higher incidence of unfavorable treatment outcome in all phases (p<0.001). Conclusion: Genomic aberrations of chromosome 20q can play an important role in the pathophysiology, development, clinical presentation, laboratory data, disease progression and treatment outcome as well as overall survival in Ph-positive CML patients.