Publications

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2020
Ahmed, W. A., F. A. - B. A. Kashwaa, O. H. A. E. M. Hameed, and M. A. Elhefny, "Potential role of Moringa oleifera on different cell lines and experimental animal model", Cancer Biology, vol. 10, issue 3, pp. 63-69, 2020. potential_role_of_moringa_oleifera_on_different_cell_lines_and_experimental_animal_model.pdf
application/pdf iconpotential_role_of_moringa_oleifera_on_different_cell_lines_and_experimental_animal_model.pdf
Aboelela, S., A. Ashmawy, S. A. B. R. Y. SHAARAWY, M. El-Hefny, and A. Medhat, "Telomerase as a Possible Candidate Targeting Therapy in Different Breast Cancer Cell Lines.", Asian Pacific journal of cancer prevention : APJCP, vol. 21, issue 8, pp. 2243-2250, 2020. Abstract
application/pdf icontelomerase_as_a_possible_candidate_targeting_therapy_in.pdf

BACKGROUND: Telomerase activity is up regulated in most breast cancer subtypes but not in the adjacent normal tissues. Thus, it is a promising target for anticancer therapy. The present work investigated the effects of telomerase inhibition by siRNA on breast cancer cell lines and studied the feasibility of whether the combined effect of doxorubicin with siRNA treatment on breast cancer cells potentiates a rapid cellular response to the cytotoxic effect of chemotherapy.  Methods: This study was performed on Luminal A (MCF-7), triple negative (MDA-MB-468), and HER-2/neu (SKBR-3) human breast cancer cell lines, wherein telomerase activity inhibition by hTERT siRNA and doxorubicin was detected by measuring telomerase activity using Telomeric Repeat Amplification Protocol (TRAP assay), assessing cell viability through MTT assay, and evaluating apoptosis through scanning electron microscopy (SEM) and through estimating caspase-3 and -8 activities using enzyme-linked immunosorbent assay (ELISA).

RESULTS: In the present study, hTERT siRNA effectively reduced telomerase activity and cell viability to more than 90% and 60%, respectively, in most breast cancer cell lines within 72 hours after transfection. The combination of hTERT siRNA and doxorubicin showed a cumulative effect compared with either treatment alone (P < 0.05). Meanwhile, SEM demonstrated apoptotic morphologic cell changes.

CONCLUSION: Telomerase inhibition is a promising strategy for the effective treatment of breast cancer. When used in combination with doxorubicin, it could potentiate the cytotoxic effect of the drug on breast cancer cells.
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2019
El‐Hefny, M., S. Fouad, T. Hussein, R. Abdel‐Hameed, H. Effat, H. Mohamed, and A. H. A. Wahab, "Circulating microRNAs as predictive biomarkers for liver disease progression of chronic hepatitis C (genotype‐4) Egyptian patients", Journal of medical virology, vol. 91, issue 1: Wiley Online Library, pp. 93-101, 2019. Abstract
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Ewis, A. A., D. M. Khalil, A. E. Arafa, Z. A. M. Salam, M. A. Elhefny, and A. El-Badawy, "Validation of a rapid screening diagnostic kit for field investigations of hepatitis C viral infection", MJMR, vol. 30, issue 3, pp. 214-219, 2019. validation_of_a_rapid_screening_diagnostic_kit_for_field_investigations_of_hepatitis_c_viral_infection.pdf
application/pdf iconvalidation_of_a_rapid_screening_diagnostic_kit_for_field_investigations_of_hepatitis_c_viral_infection.pdf
2015
A.Ahmed, W., O. - H. M.A., M. A. Elhefny, A. A. Abo Elnasr, and N. E. Syed, "The First Molecular Insights to the Effect of Ashwagandha and Propolis on Cancer cells: Comparative Study", International Conference on Advances in Agricultural, Biological & Environmental Sciences (AABES-2015) London(UK), London(UK), July 22-23, 2015. the_first_molecular_insights_to_the_effect_of_ashwagandha_and_propolis_on_cancer_cells22.pdf
application/pdf iconthe first molecular insights to the effect of ashwagandha and propolis on cancer cells
application/pdf iconthe full paper
Ahmed, W. A., M. A. Om-Hashem, M. A. Elhefny, A. A. Abo Elnasr, and N. E. Syed, "The first molecular insights to the effect of ashwagandha and propolis on cancer cells: Comparative study", medicine, vol. 28, issue 30, pp. 31, 2015. Abstract
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2014
Houseini, M. E. E. -, E. R. Amer, M. A. Elhefny, H. Mohammed, and H. H. Mohammad, "Effect of Some Diff erentiation - inducing Agents on W ild T ype Fragment - 1(Waf - 1)/P 21 and Cycline D1 (CD1) Expressions in Leukemic cells P ropagated ex - vivo", International Journal of Pharmaceutical Science and Health Care , vol. Vol. 3., issue Issue 4, , pp. 61-71, 2014. effect_of_some_diff.pdf
Wafaa A, Nasser E. A, Mohamed A. Elhefny, and D. E, "Potential toxicity of Egyptian Ashwagandha: significance for their therapeutic bioactivity and anticancer properties", Indian Journal of Natural Products and Resources (IJNPR) , vol. in press, 2014.
2013
Mohamed Abdelmoneim El-Hefny, Аrif Мostaffa Afandiyev, Sevil Karimova, and T. Dardeer, "Evaluation of Vascular Endothelial Growth Factor (VEGF) levels and Survival among Triple-Negative Breast Cancer (TNBC) and non-TNBC Cases", International Journal of Advanced Scientific and Technical Research, vol. 6, issue 3, pp. 22-36, 2013.
2012
El-Hefny, M., "Levels of vascular endothelial growth factor (VEGF) in none and triple-negative breast cancer patients ", 1st USIM International Conference on Medicine and Health (ICMH2012), Faculty of Medicine and Health Sciences, Sains University, Malizya , 19-20 may 2012.
2010
Mohamed A.El-Hefny, Arif M. Afandiyev, M. R. Quliev, and Sevil T. Karimova, "Oxidant/Antioxidant Status in Pre and Postoperative Breast Cancer Pa¬ti¬ents", The 2nd International Conference on Biological & Enviromental Sciences (Egypt), , Mansora university, 27-30 march, 2010.
Mohamed A.El-Hefny, Sevil T. Karimova, and L. R.Talisinskaya., "Investigation of Serum VEGF in breast cancer and Hepatitis C Virus Patients.", Scientific Medical Journal (EGYPT), vol. 22, issue 3, pp. 23-30, 2010.
El-Hefny M.A, Afandiev А.М, Quliyev M.R, Abelova R.G, and M. B.M., "Study of serum ceruloplasmin in breast cancer patients before and after therapy", Azerbaijan Metabolism journal, vol. 14, issue 2, pp. 39-43, 2010.
Mohamed A.El-Hefny, Sevil T. Karimova, Akif K.Hajiyev, and А. М. Afandiyev., "Studying of Serum VEGF, CEA and CA 15-3 in Pre and Postoperative Breast Cancer Patients", Medical Journal (Georgia), vol. 2, issue 1, pp. 61-64, 2010.
2009
Hefny, M. E. - A., "Study of the level of vascular endothelial growth factor in patients with breast cancer.", Conference of the 90th jubilee, , Azerbaijan medical university-Department of Human Anatomy,, 4-6 October, 2009.
Arif M. Afandiyev, Mohamed A.El-Hefny, and S. T. Karimova, "Investigation of Serum Ferritin in Breast Cancer Patients Before and After Therapy", Azerbaijan Journal of pharmacology and pharmacotherapy, vol. 2, pp. 17-23, 2009.
Mohamed A.El-Hefny, S. T. Karimova, and undefined, "Lipid Peroxidation and Antioxidant Status in Breast Cancer Patients before and after therapy", Medical Journal of Cairo University,, vol. 77, issue 3, pp. 37-42, 2009.
Afandiyev A.M., El-Hefny M.A., and K. S.T., "Oxidant/ Anti¬oxidant Status of Pre¬ope¬ra¬tive Patients with Breast Cancer. ", Azerbaijan Metabolism journal, vol. 2, issue 10, pp. 39-43, 2009.
2008
Mohamed A.El-Hefny, Afandiyev A.M., and A. G.I., "Angiogenic activity of vascular endothelial growth factor (VEGF) in breast cancer.", Azerbaijan Journal of Oncology and Hematology,, vol. 2, issue 4, pp. 45-50, 2008.
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