Afify, A., M. A. A., and H. M. M. Hassan, "Chemical composition of some plant mucilages and gums and their interactions with proteins", Conference: The Role of Biochemistry in Environment and AgricultureAt: Biochemistry Department, Faculty of Agriculture, Cairo University, EgyptVolume: 2nd Conference, Biochemistry Department, Faculty of Agriculture, Cairo University, Egypt, 2/2004. Abstract

In this study, the monosaccharides of some plant mucilages (taro mucilage, okra mucilage, Jew’s mellow mucilage and aloe vera mucilage) and gums (gum arabic, gum tragacanth, gum olibanium and gum mastic) were analyzed by HPLC as well as total uronic acids were determined. The obtained data showed that these polysaccharides are quantitatively and qualitatively differed in their contents. All mucilages and gums were contained fructose and uronic acids. The mucilages and gums were interacted with hemoglobin and altered its absorption (in range of 385-410 nm), in which a negative correlation between the absorption of Hb and the concentration of polysaccharide. In contrast, the absorption of BSA-mucilage or gum mixture was increased as the concentration of polysaccharide increase. It was demonstrated that these mucilages and gums prevent the protein precipitation (casein).

Afify, A., M. A. A., and H. M. M. Hassan, "Chemical composition of some plant mucilages and gums and their interactions with proteins", Conference: The Role of Biochemistry in Environment and AgricultureAt: Biochemistry Department, Faculty of Agriculture, Cairo University, EgyptVolume: 2nd Conference, Biochemistry Department, Faculty of Agriculture, Cairo University, Egypt, 2/2004. Abstract

In this study, the monosaccharides of some plant mucilages (taro mucilage, okra mucilage, Jew’s mellow mucilage and aloe vera mucilage) and gums (gum arabic, gum tragacanth, gum olibanium and gum mastic) were analyzed by HPLC as well as total uronic acids were determined. The obtained data showed that these polysaccharides are quantitatively and qualitatively differed in their contents. All mucilages and gums were contained fructose and uronic acids. The mucilages and gums were interacted with hemoglobin and altered its absorption (in range of 385-410 nm), in which a negative correlation between the absorption of Hb and the concentration of polysaccharide. In contrast, the absorption of BSA-mucilage or gum mixture was increased as the concentration of polysaccharide increase. It was demonstrated that these mucilages and gums prevent the protein precipitation (casein).

Gabr, G., H. Hassan, R. E. Kashef, N. Abd-Elhak, and A. Soliman, "NUTRITIONAL COMPOSITION AND BIOACTIVE COMPOUNDS CHARACTERIZATION OF PORTULACA OLERACEA L. LEAVES GROWN IN EGYPT", Fresenius Environmental Bulletin, vol. 30, issue 6, pp. 6313-6318, 2021. hazem_m.m._hassan_2021_1.pdf
Jacob, R., H. Hassan, A. Afify, and G. Gabr, "A NOVEL SYNTHESIS CHEMICAL CHARACTERIZATION AND BIOLOGICAL ACTIVITIES OF METAL-LEATHER PROTEIN HYDROLYSATE CHELATES", Fresenius Environmental Bulletin, vol. 30, issue 7, pp. 8717-8727, 2021. hazem_m.m._hassan_2021_2.pdf
Gabr, G., H. M. M. Hassan, V. D. Seshadri, and N. Hassan, "Comparative study of phenolic profile, antioxidant and antimicrobial activities of aqueous extract of white and green tea", Zeitschrift fur Naturforschung C, 2022. Abstract

The sole difference between white tea (WT) and green tea (GT) is the former that made only from the buds and young leaves of the Camelia sinensis plant, whilst the latter is made from matured tea leaves. The phytochemical profiles, phenolic compounds, antioxidant, and antimicrobial activity of two varieties of Camellia sinensis teas, white and green, were compared in this study. Total antioxidant capacity, reducing power, DPPH radical scavenging, and Fe+2 chelating activities were used to determine antioxidant activities in water extract of GT and WT. The largest level of phenolic content was discovered in WGTE compared with the lowest amount was found in WWTE (290.67 mg/100 g tea and 185.96 mg/100 g tea, respectively). Phenoilc acids (gallic, benzoic, chlorogenic, ellagic, and ρ-coumaric acids) and flavonoids (rutin and kampherol) were found in the two extracts. The findings of DPPH radical scavenging assays were 84.06 and 82.37% inhibition. In vitro antimicrobial activity was indicated that (WWTE and WGTE) had a high level of activity against Staphylococcus aureus, and gave negative activity against Salmonella typhimurium, and Aspergillus Niger. The WT and GT extracts are a great source of natural antioxidants with biological effects on human health.

Gabr, G., H. Hassan, R. E. Kashef, N. Abd-Elhak, and A. Soliman, "CYTOTOXIC, ANTIMICROBIAL AND BRAIN PROTECTIVE EFFECT OF BIOACTIVE PHENOLIC COMPOUNDS PRODUCED FROM Portulaca oleracea L.", Fresenius Environmental Bulletin, vol. 31, issue 01A, pp. 971-978, 2022. hassan_et_al._2022.pdf
Jacob, R. H., H. M. M. Hassan, and A. S. Afify, "Evaluation Of Antioxidant And Metal Chelating Activities Of Protein Hydrolysates Produced From Leather Waste By Alkaline And Enzymatic Hydrolysis", Research Journal of Pharmaceutical, Biological and Chemical Sciences, vol. 7, issue 4, pp. 910-919, 2016. 116.pdf
Shallan, M. A., H. M. M. Hassan, A. A. M. Namich, and A. A. Ibrahim, "Biochemical and Physiological Effects of TiO2 and SiO2 Nanoparticles on Cotton Plant under Drought Stress", Research Journal of Pharmaceutical, Biological and Chemical Sciences, vol. 7, issue 4, pp. 1540-1551, 2016. 200.pdf
Shallan, M. A., H. M. M. Hassan, A. A. M. Namich, and A. A. Ibrahim, "The Influence of Lithovit Fertilizer on the Chemical Constituents and Yield Characteristics of Cotton Plant under Drought Stress", International Journal of ChemTech Research, vol. 9, issue 8, pp. 1-11, 2016. Hazem.pdf
Afify, A. E. - M. M. R., and H. M. M. Hassan, "Free radical scavenging activity of three different flowers-Hibiscus rosa-sinensis, Quisqualis indica and Senna surattensis", Asian Pacific Journal of Tropical Biomedicine, vol. 6, issue 9, pp. 771-777, 2016. Hazem .pdf
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