YASSER ABDELRAHMAN

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Yasser M. Abdelrahman

EDUCATION

• Ph.D.

  in Microbiology, University of Tennessee Health Science Center in Oct. 2009. Title of Ph.D. Thesis “Discovery and Validation of New Regulatory RNA Elements in Chlamydia trachomatis”

• Graduate PhD Program

  in the Microbiology, Immunology and Biochemistry Dept. University of Tennessee Health Science Center, Tennessee USA 2004-2009.

• Graduate Internship

  in the VA Medical Center, Memphis, Tennessee USA 2001-2003.

• M.Sc.

  in Pharmacy and

  Pharmaceutical Sciences, in Aug. 2001. Title of M.Sc. Thesis” Development of new rapid serological tests for Human Fascioliasis”

• Graduate M.Sc.Program

  in the Microbiology and Immunology Dept. faculty of Pharmacy, Cairo University 1997-2001.

• UNDERGRADUATE:

  B.Sc. of Pharmacy and

  Pharmaceutical Sciences, Faculty of Pharmacy, Cairo University, Cairo, Egypt 1997.

RESEARCH EXPERIENCE

• University of Tennessee HSC Department of Microbiology Immunology and Biochemistry

  Aug. 2010-present.

              Mentor

: Dr. Robert Belland and Dr. John Cox

• Study: We study the interaction of chlamydia with host lipids and how chlamydia utilizes the host high density lipoprotein (HDL) biosynthetic machinery to acquire host-derived phospholipids. 
• Study: We are evaluating a new FabI inhibitor as a potential narrow spectrum antibiotic that targets a small fraction of the human microbiota.
• Study: Studying the unique process of differentiation of Chlamydia early in its developmental cycle.

• University of Tennessee HSC Department of Molecular Science

  2004-2009.

      Mentor

: Dr. Robert Belland

• Study: Discovery and Validation of New Regulatory RNA Elements in C. trachomatis. An intergenic microarray was designed and used to discover new RNA elements expressed within the intergenic regions, these new RNA elements were mapped and their expression was validated under normal and stressed growth conditions.

• Memphis VA Medical Center

  2001-2003.

: Dr. Malak Kotb

• Study: Cloning, expression and purification of MAT-FLAG fusion protein, the purified protein was then used for LCMS analysis.
• Study: Cloning of lentiviral vectors and transfecting human islets of Langerhans.

• Cairo University Department of Microbiology and Immunology

  1997-2001.

Mentor

: Dr. Hussein Shoeb

• Study: Development and evaluation of rapid serological tests for human fascioliasis. A novel latex agglutination reagent and a lateral flow device were developed and tested for accuracy and specificity

LABOEATORY TECHNIQUES

RNA/DNA extraction, 5’ and 3’RACE, RNA circularization, Quantitative PCR, RT-PCR, Northern and Western Blotting, Labeling RNA probes, SDS and Native PAGE, Small RNA analysis, General cloning, Cloning toxic genes, Mutagenesis, Cell culture techniques, Bioinformatics, Microarray analysis, Library construction and screening, Sequencing, Immunohistochemistry, Microscopy (confocal, deconvolution), Flow Cytometery, Transformation, Transfection, General lab protocols e.g. plasmid preps, electrophoresis, PCR.

PUBLICATIONS & PRESENTATIONS

• Publications

1. Abdelrahman Y,

   Ouellette SP, Belland RJ, Cox JV. 2016. Polarized Cell Division of Chlamydia trachomatis. PLoS Pathog

   12:

   e1005822.

2. Abdelrahman Y,

   Ouellette SP, Belland RJ, Cox JV

   .

   2016. Correction: Polarized Cell Division of Chlamydia trachomatis. PLoS Pathog

   12:

   e1005866.

3. Ouellette SP, Rueden KJ,
   AbdelRahman YM,

   Cox JV, Belland RJ. 2015. Identification and Partial Characterization of Potential FtsL and FtsQ Homologs of Chlamydia. Front Microbiol

   6:

   1264.

4. Bruhn DF, Waidyarachchi SL, Madhura DB, Shcherbakov D, Zheng Z, Liu J,
   Abdelrahman YM,

   Singh AP, Duscha S, Rathi C, Lee RB, Belland RJ, Meibohm B, Rosch JW, Bottger EC, Lee RE

   .

   2015. Aminomethyl spectinomycins as therapeutics for drug-resistant respiratory tract and sexually transmitted bacterial infections. Sci Transl Med

   7:

   288ra275.

5. Yao J,
   Abdelrahman YM,

   Robertson RM, Cox JV, Belland RJ, White SW, Rock CO. 2014. Type II fatty acid synthesis is essential for the replication of Chlamydia trachomatis. J Biol Chem

   289:

   22365-22376.

6. Lewis ME, Belland RJ,
   AbdelRahman YM,

   Beatty WL, Aiyar AA, Zea AH, Greene SJ, Marrero L, Buckner LR, Tate DJ, McGowin CL, Kozlowski PA, O'Brien M, Lillis RA, Martin DH, Quayle AJ. 2014. Morphologic and molecular evaluation of Chlamydia trachomatis growth in human endocervix reveals distinct growth patterns. Front Cell Infect Microbiol

   4:

   71.

7. Frazer LC, Darville T, Chandra-Kuntal K, Andrews CW, Jr., Zurenski M, Mintus M,
   AbdelRahman YM,

   Belland RJ, Ingalls RR, O'Connell CM

   .

   2012. Plasmid-cured Chlamydia caviae activates TLR2-dependent signaling and retains virulence in the guinea pig model of genital tract infection. PLoS One

   7:

   e30747.

8. Cox JV, Naher N,
   Abdelrahman YM,

   Belland RJ. 2012. Host HDL biogenesis machinery is recruited to the inclusion of Chlamydia trachomatis-infected cells and regulates chlamydial growth. Cell Microbiol

   14:

   1497-1512.

9. O'Connell CM,
   AbdelRahman YM,

   Green E, Darville HK, Saira K, Smith B, Darville T, Scurlock AM, Meyer CR, Belland RJ.

   2011. Toll-like receptor 2 activation by Chlamydia trachomatis is plasmid dependent, and plasmid-responsive chromosomal loci are coordinately regulated in response to glucose limitation by C. trachomatis but not by C. muridarum. Infect Immun

   79:

   1044-1056.

10. Ibana JA, Belland RJ, Zea AH, Schust DJ, Nagamatsu T,
    AbdelRahman YM,

    Tate DJ, Beatty WL, Aiyar AA, Quayle AJ

    .

    2011. Inhibition of indoleamine 2,3-dioxygenase activity by levo-1-methyl tryptophan blocks gamma interferon-induced Chlamydia trachomatis persistence in human epithelial cells. Infect Immun

    79:

    4425-4437 

11. Abdelrahman YM,

    Rose LA, Belland RJ.

    2011. Developmental expression of non-coding RNAs in Chlamydia trachomatis during normal and persistent growth. Nucleic Acids Res

    39:

    1843-1854.

12. Ouellette SP, Hatch TP,
    AbdelRahman YM,

    Rose LA, Belland RJ, Byrne GI. 2006. Global transcriptional upregulation in the absence of increased translation in Chlamydia during IFNgamma-mediated host cell tryptophan starvation. Mol Microbiol

    62:

    1387-1401.

13. Ouellette SP,
    Abdelrahman YM,

    Belland RJ, Byrne GI

    .

    2005. The Chlamydia pneumoniae type III secretion-related lcrH gene clusters are developmentally expressed operons. J Bacteriol

    187:

    7853-7856.

14. Abdelrahman YM,

    Belland RJ.

    2005. The chlamydial developmental cycle. FEMS Microbiol Rev

    29:

    949-959. 

• Presentations:

1. 12^th Euro Fed Lipid Congress at Montpellier, France on September 14-17 2014, “ Endogenous phosphatidylethanolamine synthesis using fatty acids made from FASII is required for Chlamydia trachomatis replication

   .”

1. 5^th Chlamydia Basic research meeting oral presentation at Redondo Beach CA on March 18-21, 2011 Titled “ Sequential Positive and Negative Selection to Identify Targets of Non-Coding RNAs

   ”

1. 3^rd Chlamydia Basic research meeting oral presentation at Louisville KY March 23-26, 2007 Titled “ Developmental Expression of ncRNA in Chlamydia trachomatis, A Role in Persistence?

   ”

1. 2^nd Chlamydia Basic research meeting oral presentation at Indianapolis IN April 23-25, 2005 Titled “ Transcriptional Analysis of Differentially Processed Chlamydial mRNAs: Use of Intergenic Microarrays

   ”

PROFESSIONAL EXPERIENCE

• Post-doctoral trainee

  University of Tennessee HSC in Memphis Aug 2010-present.

• Medical Representative

  Boehringer Ingelheim Medical Office 1998-2000. Cairo, Egypt

• Licensed Pharmacist

  1997-present. Cairo, Egypt.

• Intern Pharmacist

  1992-1997. Cairo, Egypt.

TEACHING EXPERIENCE

• Graduate teaching assistant

  University of Tennessee HSC in Memphis 2004-2009. Participated in several practical labs for Medical students under the mentorship of Dr. Tomas Hatch

• Assistant lecturer

  in the Microbiology and Immunology Department faculty of Pharmacy, Cairo University 2001. Cairo, Egypt. Lab coordinator for second and third year pharmacy students, where I was personally responsible for 300 students from each grad.

• Instructor and research assistant

  in the Microbiology and Immunology Department faculty of Pharmacy, Cairo University 1997. Cairo, Egypt. Conducted practical sections for second and third year pharmacy students, involving teaching the following courses: General Microbiology, Pharmaceutical Microbiology, Medical Microbiology, Parasitological, Microbiological methods for Antibiotic assay, Microbiological analysis of Milk and Water, Serological detection of microbes, T.B. detection and acid fast staining

   

AWARDS AND HONORS

• Graduated from College of Pharmacy, Cairo University, Excellent (87.4%) with honors ranking Seventh College wide. May 1997.

• Novartis Pharma Industrial Plant honor Certificate, Cairo, Egypt 1995.

• Misr Pharmaceutical Plant Q.C. internship Certificate, Cairo, Egypt 1995.

• ACCA Industrial Plant internship Certificate, 6^th of October, Egypt 1994.

• Graduated from high school with honors ranking Sixth (91.75%) school wide June 1992.

• Board Certificate

  :  

  • Certified Instructor and Lecturer in the Department of Microbiology and Immunology Faculty of Pharmacy Cairo University. Cairo, Egypt 2010.
  • Arab Republic of Egypt Board of Pharmacy 1997.

• Society Membership:
  • Chlamydia Basic Research Society, USA 2005.
  • American Society of Microbiology, USA 2001.
  • Syndicate of Pharmacy Cairo, Egypt 1997

(Last updated: Sat, 2017-03-25 00:37)