Characterization, kinetics and thermodynamics of biosynthesized uranium nanoparticles (UNPs), Abostate, Mervate Aly, Saleh Youssry, Mira Hamed, Amin Maysa, Kazindar Maha Al, and Ahmed Basma Mahmoud , Artificial Cells, Nanomedicine, and Biotechnology , Volume 46, Issue 1, p.147-159, (2018) Abstract

The present study was carried out to explore the potential of the isolated bacterial strains isolated from Gabal El Sela in Eastern Dessert, Egypt for biosynthesis of uraninite nanoparticles intracellularly. The most potent bacterial strains associated (intra) with uranium nanoparticles were characterized by transmission electron microscope (TEM), Fourier transform infrared (FTIR) and Energy Dispersive X-ray (EDX). Studying factors affecting biosynthesis of uranium nanoparticles indicated that the optimum conditions were 6000 ppm uranium concentrations at pH 7.0 and temperature 30 °C ± 1 after five days with 10% biomass under shaking conditions and the maximum uranium uptake by MAM – U9 cells was 3300 ppm (55%) from uranyl nitrate solution and 3600 ppm (72%) from Sela rock sample. Results of TEM micrograph show those uranium nanoparticles (UNPs) with size ranging from 2.9 to 21.13 nm inside cells. The kinetics, isotherm and thermodynamics parameters of uranium uptaken by bacterial strain MAM –U9 have been determined and found to be a first order process (R2 = 9935), follows Langmuir isotherm (RL2 = 0.998) and the thermodynamics of ΔG = –9.715 kJmol−1, ΔH = 16.987 kJmol−1 and ΔS = 0.0881 kJmol−1 K−1 at 30 °C.

Decolorization of Congo Red dye by bacterial isolates, Abo-State, M. A. M., Saleh Y. E., and Hazaa H. A. , J.Ecol.Heal.Environ., Volume 5 (2), p.41 - 48, (2017) Abstract

Congo Red (CR) is one of the azo dyes which is widely used in textile industries and has carcinogenic properties. Large amount of wastewater containing these dyes is discharged from the printing units causing an environmental pollution. So, it is very important to degrade these dyes before discharging it. Several bacterial strains isolated from textile wastewater were tested to study its potential to decolorize CR dye. The decolorization of Congo Red dye by the bacterial strains was observed in various concentrations (25 to 400 mg/L) of CR dye. The most potent bacterial strains were B. cereus MAM-B22, Ochrobactrum sp. MAM-C9, Achromobacter xylosoxidans MAM-29 and B. cereus MAM-B11. The maximum decolorization was observed by B. cereus MAM-B22 which removed 96.92%, 93.34%, 89.28%, 82.51%, 75.57%, 69.18% and 66.73% of 25, 50, 100, 150, 200, 300 and 400 mg/L respectively after 3 days of incubation at 37°C. When different pH values were used, the maximum decolorization was observed at pH 7.0 which was 90.21%, 90.03%, 89.62% and 85.84% by Ochrobacterum sp. MAM-C9, B. cereus MAM-B22, A. xylosoxidans MAM-29 and B. cereus MAM-B11 respectively. The effect of temperature was studied at a range of 25-60°C and the optimum temperature was 37°C. The percentage of decolorization of CR was 92.56%, 91.37%, 89.29% and 88.69% by B. cereus MAM-B22, Ochrobacterum sp. MAM-C9, A. xylosoxidans MAM-29 and B. cereus MAM-B11 respectively. The decolorization percentage was bacterial strain, concentration and time dependent.

Aminoglycoside and beta-lactate resistance pattern among Gram-negative bacilli (GNB) isolated from Egyptian hospitals between 2012-2014, Saleh, Y. E., Abo-State M. A. M., Helal N. L., and Gareeb H. M. , J.Eco.Heal.., Volume 2, p.61 - 65, (2016)
Efficacy of Antibiotic Combinations on multi-drug Resistant Bacterial Strains Isolated from Urinary Tract Infection and Heodialysis Patient, Abo-State, M. A. M., Saleh Y. E., and Fathy S. , J. Eco. Heal.Env., Volume 4 , Issue 2, Cairo University, p.61 - 65, (2016)
Introduction to Bacteriology 2nd. edition, Saleh, Youssry , Cairo University, (2016)
General Microbiology, Saleh, Youssry , Cairo University, (2016)