Elshaghabee, F. M. F., A. El-Hussein, and M. S. M. Mohamed, "Enhancement of Labneh Quality by Laser-Induced Modulation of Lactocaseibacillus casei NRRL B-1922", Fermentation, vol. 8, issue 3, 2022.
KHEDR, A., H. Imam, A. M. Alhajoj, and A. El-Hussein, "Identification and differentiation between olive oil from different Al Jouf olive field areas by optical spectroscopic techniques", AIP Advances, vol. 12, issue 10, 2022.
Altowyan, A., A. El-Hussein, H. A. Ahmed, H. Hassan, A. Ameen, A. Mostafa, and M. Elfaham, "Influence of the laser wavelength on the self-absorption of Cu and Ni spectral lines by using LIBS technique", Optical Materials, vol. 131, 2022.
application/pdf iconoptical_materials_pdf.pdf
El-Hussein, A., S. L. Manoto, S. Ombinda-Lemboumba, Z. A. Alrowaili, and P. Mthunzi-Kufa, "A Review of Chemotherapy and Photodynamic Therapy for Lung Cancer Treatment.", Anti-cancer agents in medicinal chemistry, 2021. Abstract

Cancer is among the leading causes of mortality and morbidity worldwide. Among the different types of cancers, lung cancer is the considered to be the leading cause of death related to cancer and the most commonly diagnosed form of such disease. Chemotherapy remains a dominant treatment modality for many types of cancers at different stages. However, in many cases cancer cells develop drug resistance and become non-response to chemotherapy; thus necessitating the exploration of alternative and /or complementary treatment modalities. Photodynamic Therapy (PDT) has emerged as an effective treatment modality for various malignant neoplasia and tumors. In PDT, the photochemical interaction of light, Photosensitizer (PS) and molecular oxygen produces Reactive Oxygen Species (ROS) which induces cell death. Combination therapy by using PDT and chemotherapy can promote synergistic effect against this fatal disease with the elimination of drug resistance, and enhancement the efficacy of cancer eradication. In this review, we give an overview of chemotherapeutic modalities, PDT and the different types of drugs associated with each therapy. Furthermore, we also explored the combined use of chemotherapy and PDT in the course of lung cancer treatment and how this approach could be the last resort for thousands of patients that have been diagnosed by this fatal disease.

N.Houreld, N., and A. El-Hussein, "Chapter 11 - Photodynamic therapy-based tuberculosis treatment Author links open overlay panel", A Mechanistic Approach to Medicines for Tuberculosis Nanotherapy: Elsevier, 2021.
El-Hussein, A., and M. Kasem, "Exploiting FTIR microspectroscopy and chemometric analysis in the discrimination between Egyptian ancient bones: a case study", Journal of the Optical Society of America B, vol. 37, issue 11, pp. A110-A120, 2020.
Abdelsalam, E., A. El-Hussein, and M. Samer, "Photobiostimulation of anaerobic digestion by laser irradiation and photocatalytic effects of trace metals and nanomaterials on biogas production", International Journal of Energy Research, 2020.
El-Hussein, A., M. R. Hamblin, A. Saad, and M. A. Harith, "Photobiomodulation of avian embryos by red laser.", Lasers in medical science, 2020. Abstract

The current research focuses on the effect of variable doses of red laser on the chick embryonic development. He-Ne laser of 632-nm wavelength was used as an irradiation source in the first 48 h post-laying of chicken eggs. We have used five different doses: 2, 1, 0.3, 0.2, and 0.1 mJ/cm that needed a time range for about 400-20 s. Those irradiated embryos were left for additional 11 days for incubation in normal conditions, where they are blindly studied after the 11 day. Light microscopy was used in this study to investigate the histological and pathological features of the different experimental groups compared to the control one. However, electron microcopy was utilized to trace the apoptotic distribution in the developmental embryos. Minor abnormalities that are dependent on the laser dose have been shown in the irradiated embryos when compared to the sham group, where the highest laser dose showed about 12% embryonic development anomalies when related to the other irradiated groups. Irradiated embryos were found to express more INF-γ and IL-2 as circulating cytokines relative to the unexposed group, where the levels of IL-2 were highly significantly increased by all laser doses (0.3 mJ/cm light dose recipient group showed significant increase only when compared to the control group). IFN-γ levels were significantly increased as well by light doses above 0.2 mJ/cm. This IFN-γ increase trend seemed to be laser dose-dependent. Simultaneously, these combined results propose the ability of high laser doses in inducing incurable changes in the embryonic development and consequently such alterations can have potential therapeutic applications through what is known as photobiomodulation.

Manoto, S. L., A. El-Hussein, R. Malabi, L. Thobakgale, S. Ombinda-Lemboumba, Y. A. Attia, M. A. Kasem, and P. Mthunzi-Kufa, "Exploring optical spectroscopic techniques and nanomaterials for virus detection.", Saudi journal of biological sciences, 2020. Abstract

Viral infections pose significant health challenges globally by affecting millions of people worldwide and consequently resulting in a negative impact on both socioeconomic development and health. Corona virus disease 2019 (COVID-19) is a clear example of how a virus can have a global impact in the society and has demonstrated the limitations of detection and diagnostic capabilities globally. Another virus which has posed serious threats to world health is the human immunodeficiency virus (HIV) which is a lentivirus of the retroviridae family responsible for causing acquired immunodeficiency syndrome (AIDS). Even though there has been a significant progress in the HIV biosensing over the past years, there is still a great need for the development of point of care (POC) biosensors that are affordable, robust, portable, easy to use and sensitive enough to provide accurate results to enable clinical decision making. The aim of this study was to present a proof of concept for detecting HIV-1 pseudoviruses by using anti-HIV1 gp41 antibodies as capturing antibodies. In our study, glass substrates were treated with a uniform layer of silane in order to immobilize HIV gp41 antibodies on their surfaces. Thereafter, the HIV pseudovirus was added to the treated substrates followed by addition of anti-HIV gp41 antibodies conjugated to selenium nanoparticle (SeNPs) and gold nanoclusters (AuNCs). The conjugation of SeNPs and AuNCs to anti-HIV gp41 antibodies was characterized using UV-vis spectroscopy, transmission electron microscopy (TEM) and zeta potential while the surface morphology was characterized by fluorescence microscopy, atomic force microscopy (AFM) and Raman spectroscopy. The UV-vis and zeta potential results showed that there was successful conjugation of SeNPs and AuNCs to anti-HIV gp41 antibodies and fluorescence microscopy showed that antibodies immobilized on glass substrates were able to capture intact HIV pseudoviruses. Furthermore, AFM also confirmed the capturing HIV pseudoviruses and we were able to differentiate between substrates with and without the HIV pseudoviruses. Raman spectroscopy confirmed the presence of biomolecules related to HIV and therefore this system has potential in HIV biosensing applications.

Tourism