Elemam, N. M., R. Y. Mekky, N. M. El-Ekiaby, S. A. E. Sobky, M. A. M. E. Din, G. Esmat, and A. I. Abdelaziz, "Repressing PU.1 by miR-29a∗ in \{NK\} cells of \{HCV\} patients, diminishes its cytolytic effect on \{HCV\} infected cell models", Human Immunology, pp. -, 2015. AbstractWebsite

AbstractObjectives Natural killer cells are immune safeguards against \{HCV\} infection. PU.1 is a pivotal transcription factor in the development of \{NK\} cells. This study aimed at studying the regulatory effect of miRNAs on both development and function of \{NK\} cells isolated from \{HCV\} patients. Methods \{NK\} cells were isolated from 17 chronic \{HCV\} patients and 12 healthy controls; after which miRNA and mRNA were quantified using qRT-PCR. Manipulating miRNA expression using mimics and antagomirs, was performed followed by investigating downstream targets as well as viral abundance. Results PU.1 expression levels were upregulated in \{NK\} cells of \{HCV\} patients. In silico analysis revealed PU.1 to be a potential downstream target of miR-29a∗, where miR-29a∗ overexpression in \{NK\} cells caused a significant downregulation in PU.1 mRNA. Forcing miR-29a∗ caused a downregulation of the cytotoxicity determinant \{NK\} activating receptor (NKG2D) via upregulation of miR-155. Moreover, perforin-1 mRNA was found to be downregulated upon forcing the expression of miR-29a∗ in \{NK\} cells of \{HCV\} patients. This decrease in \{NK\} cytolytic function was accompanied by an 80% viral load increase in cocultured \{HCVcc\} cell models. Conclusions This study showed that \{HCV\} infection might abrogate \{NK\} cytotoxic potential through altering PU.1, \{NKG2D\} receptor and perforin molecules.

doaa ghaith, M. Elzahry, G. Mostafa, S. Mostafa, R. E. Sherif, and I. Ramzy, "Mutations affecting domain V of the 23S rRNA gene in Helicobacter pylori from Cairo, Egypt.", Journal of chemotherapy (Florence, Italy), pp. 1973947815Y0000000067, 2015 Sep 11. Abstract

BACKGROUND: Clarithromycin is a main component of the recommended first-line triple therapy for Helicobacter pylori in Egypt. We aimed in our study to investigate the prevalence of clarithromycin-resistant H. pylori strains due to the point mutations at domain V of the H. pylori 23S rRNA among the Egyptian population using the polymerase chain reaction/restricted fragment length polymorphism (PCR/RFLP) assay.

METHODS: Gastric biopsies obtained from 100 dyspeptic patients who consecutively attended at Cairo University Hospital during the period from January to November 2013 were subjected to PCR/RFLP in order to detect the point mutations at domain V of the H. pylori 23S rRNA associated with clarithromycin resistance. The PCR amplicon of the 23S H. pylori rRNA is restricted with MboII for detection of A2142G mutation and with BsaI for A2143G mutation.

RESULTS: The prevalence of H. pylori infection among 100 patients was 70%; clarithromycin resistance was detected in 39/70 (57.7%) of positive H. pylori isolates. Occurrence of 23S rRNA A2142G mutations resulted in two DNA fragments (418 and 350 bp) by PCR-RFLP; on the other hand, no A2143G mutations were detected.

CONCLUSIONS: The high prevalence of clarithromycin resistance (57.7%) caused by A2142G mutations at domain V of the H. pylori 23S rRNA may mandate changing of the standard clarithromycin-containing triple therapy. The PCR/RFLP assay was a rapid and accurate method for molecular detection of H. pylori infection in addition to determination of different nucleotide mutations causing clarithromycin resistance.

Bahgat, N. A., M. M. Kamal, A. O. Abdelaziz, M. A. Mohye, H. I. Shousha, M. M. Ahmed, T. M. Elbaz, and M. M. Nabil, "Interferon-$\gamma$ and Interleukin-10 Gene Polymorphisms are not Predictors of Chronic Hepatitis C (Genotype-4) Disease Progression.", Asian Pacific journal of cancer prevention: APJCP, vol. 16, no. 12, pp. 5025–5030, 2015. Abstract
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, "ORAL PRESENTATIONS", Journal of Hepatology, vol. 60, no. 1 Supplement, pp. S121-S122, 2014. Abstract

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Khattab, H., A. Fouad, M. Hamza, M. A. Mohey, W. El-Akel, H. Ghoneim, A. Abul-Fotouh, and G. Esmat, "Relation of ALT and AST levels to the histopathological changes in liver biopsies of patients with chronic hepatitis C genotype 4", Arab Journal of Gastroenterology: Elsevier, 2015. Abstract
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Shehab, H., I. Elattar, T. Elbaz, M. Mohey, and G. Esmat, "CUFA algorithm: assessment of liver fibrosis using routine laboratory data", Journal of viral hepatitis, vol. 21, issue 12, pp. 956-964, 2014. Abstract
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Hassan, E. M., O. G. Shaker, M. A. S. Bary, H. Ghoneim, M. A. Mohey, R. N. Marzaban, and L. A. Hafez, "Biliary and Serum Insulin-Like Growth Factor-I: Are They Reliable Diagnostic Markers in Cholangiocarcinoma?", World Journal of Medical Sciences, vol. 11, issue 2, pp. 155-160, 2014. Abstract
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Riad, S. E., N. El‑Ekiaby, R. Y. Mekky, R. Ahmed, M. Eldin, M. Ahmed, M. El‑Sayed, M. M. Abouelkhair, A. Salah, and A. R. Zekri, "Expression signature of microRNA‑155 in hepatitis C virus genotype 4 infection", Biomedical reports, vol. 3, issue 1: Spandidos Publications, pp. 93-97, 2015. Abstract
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Elhelw, D. S., R. Y. Mekky, N. El‑Ekiaby, R. Ahmed, M. Eldin, M. Ahmed, M. El‑Sayed, M. M. Abouelkhair, A. Salah, and A. R. Zekri, "Predictive prognostic role of miR‑181a with discrepancy in the liver and serum of genotype 4 hepatitis C virus patients", Biomedical reports, vol. 2, issue 6: Spandidos Publications, pp. 843-848, 2014. Abstract
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Zakaria, M. S., I. M. Hamza, M. A. Mohey, and R. G. Hubamnn, "The first Egyptian experience using new self-expandable metal stents in acute esophageal variceal bleeding: Pilot study", Saudi Journal of Gastroenterology, vol. 19, issue 4: Medknow Publications, pp. 177, 2013. Abstract
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