Khalil, R. R., and M. M. Y. Madany, "Stigmasterol relieves the deleterious effects of copper stress in maize", Egyptian Journal of Experimental Biology, vol. 13, pp. 99-110, 2017. stigmasterol.pdf
Madany, M. M. Y., and A. M. Saleh, "Phytotoxicity of Euphorbia helioscopia L. on Triticum aestivum L. and Pisum sativum L.", Annals of Agricultural Sciences, vol. 60, issue 1, pp. 141-151, 2015. 2015_3-annals_of_agricultural_sciences.pdf
Madany, M. M. Y., and A. M. Saleh, "Phytotoxicity of Euphorbia helioscopia L. on Triticum aestivum L. and Pisum sativum L.", Annals of Agricultural Science, vol. 60, issue 1, pp. 141-151, 2015. AbstractFULL TEXT.pdf

Invasive field weeds, such as Euphorbia sp., represent main threat for crop productivity. The present work was conducted to evaluate the phytotoxicity of Euphorbia helioscopia on wheat (Triticum aestivum L.) and pea (Pisum sativum L.). The influence of aqueous extract from shoot of E. helioscopia, at different rates (1.0%, 2.5%, 5.0%, and 10.0%; w/v), on germination and early seedling growth of wheat and pea as well as some of the synchronized physiological aspects was investigated. E. helioscopia aqueous extract severely affected the germination in a concentration dependent manner. Plumule and radicle length, as well as their fresh and dry masses were markedly reduced. Moreover, amylase activity and total soluble sugars were significantly reduced in response to treatment with aqueous Euphorbia extract, in both test plants, whereas, proteolytic activity showed marked improvement. The linear regression analysis revealed the presence of positive linear correlation between germination rate and amylase activity. The stress markers such as proline, phe- nolics and flavonoids were markedly accumulated upon treatment with E. helioscopia extract. The increment in the level of total phenolics was concomitant with an improvement in phenylalanine ammonia lyase and peroxidase activities. High performance liquid chromatography analysis of E. helioscopia extract revealed the presence of two cinnamic acid derivatives (caffeic and p-coumaric acids), two benzoic acid derivatives (vanillic and syringic acids) and a flavanonol (Dihydroquercetin). To our knowledge, this is the first study to investigate the physiological implications underlying the phytotoxicity of E. helioscopia.

Saleh, A. M., and M. M. M. Y. Department, "Coumarin pretreatment alleviates salinity stress in wheat seedlings", Plant Physiology and Biochemistry, vol. 88, pp. 27-35, 2015. Abstract

The potentiality of COU to improve plant tolerance to salinity was investigated. Wheat grains were primed with COU (50 ppm) and then grown under different levels of NaCl (50, 100, 150 mM) for two weeks. COU pretreatment improved the growth of wheat seedling under salinity, relative to COU- untreated seedlings, due to the accumulation of osmolytes such as soluble sugars and proline. More- over, COU treatment significantly improved Kþ/Naþ ratio in the shoots of both salt stressed and un- stressed seedlings. However, in the roots, this ratio increased only under non-salinity. In consistent with phenylalanine ammonia lyase (PAL), phenolics and flavonoids were accumulated in COU-pretreated seedlings under the higher doses of salinity, relative to COU-untreated seedlings. COU primed seedlings showed higher content of the coumarin derivative, scopoletin, and salicylic, chlorogenic, syringic, vanillic, gallic and ferulic acids, under both salinity and non-salinity conditions. Salinity stress signifi- cantly improved the activity of peroxidase (POD) in COU-pretreated seedlings. However, the effect of COU on the total antioxidant capacity (TAC) was only obtained at the highest dose of NaCl (150 mM). The present results suggest that COU pretreatment could alleviate the adverse effect of salinity on the growth of wheat seedlings through enhancing, at least partly, the osmoregulation process and antioxidant defense system.

Al-Wakeel, S. A. M., H. Moubasher, M. M. A. Gabr, and M. M. Y. Madany, "Induction of Systemic Resistance in Tomato Plants against Orobanche ramosa L. Using Hormonal Inducers", Egyptian journal of Botany, vol. 52, no. 2, pp. 1–17, 2012. Abstract

Branched broomrape (Orobanche ramosa L.) represents a major constraint for tomato (Lycopersicum esculentum Mill.) production in the Mediterranean region. The efficiency of both salicylic acid (SA) and indole acetic acid (IAA) to induce resistance of tomato against broomrape was evaluated under controlled conditions. Priming of tomato seeds with different concentrations of either SA or IAA significantly reduced the number and biomass of Orobanche tubercles that accompanied by improvement of tomato root growth, where the effects are concentration-dependent. The reduction in chlorophyll a and b in the tomato leaf tissues due to Orobanche infection was improved upon application of both elicitors. Likewise, SA and IAA treatments elevated the level of total soluble sugars in tomato roots in the healthy and infected plants. The induced resistance of tomato in response to each elicitor was correlated to the increase of lignification in root tissues accompanied by enhancement in the activity of chitinase and ~-1, 3-glucanase that was higher in the infected- than healthy plants. These findings suggest that SA and IAA can activate defense mechanisms

Al-Wakeel, S. A. M., H. Moubasher, M. M. A. Gabr, and M. M. Y. Madany, "Induced systemic resistance : an innovative control method to manage branched broomrape ( Orobanche ramosa L .) in tomato", IUFS Journal of Biology, vol. 72, no. 1, pp. 9–21, 2013. AbstractWebsite

The biochemical responses of tomato (Lycopersicon esculentum Mill.) roots to different concentrations of the hormonal elicitors, salicylic acid (SA) and indole acetic acid (IAA), as presoaking treatments, on the healthy and Orobanche ramosa-infected plants were studied in pot experiment under control conditions. Application of SA and IAA activate the production of proline and soluble proteins in tomato roots, where their levels were more pronounced in the infected than the healthy plants. Moreover, both elicitors increased the levels of phenolics and flavonoids accompanied by stimulations in the activities of phenylalanine ammonia lyase (PAL), peroxidase (POX) and polyphenol oxidase (PPO) in tomato roots, where the magnitude of induction were more obvious in infected plants than healthy one. Also, microscopic examination revealed the anatomical differences observed in both untreated healthy and infected plants as well as in plants treated with SA and IAA. The present results indicate the effectiveness of SA and IAA in enhancing resistance of tomato to retard the incidence of infestation by Orobanche. These biochemical and histological changes involved in plant defense mechanisms may be one of the basic ways participate in the action of SA and IAA for increasing resistance in tomato against Orobanche ramosa.
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Saleh, A. M., and M. Madany, "Investigation of the Allelopathic Potential of Alhagi graecorum", Asian Journal of Agricultural Research, vol. 8, no. 1, pp. 42–50, 2014. Abstract

The current study evaluated the allelopathic potential of Alhagi gra.ecorum on germination and
seedling growth of two common crop plants; bean (Vicia faba) and corn (Zea mays). Water soluble allelochemicals were extracted from the air dried -powdered shoots of A. gra.ecorum at three different concentrations (2.0, 4.0 and 6.0%, w/v). The germination experiment revealed that seeds of both bean and corn have tolerance to the aqueous extract of A. graecorum, where concentrations up to 6.0% had no significant effect on percent of germination as compared with the untreated seeds. The results showed that the lowest concentration (2.0%) of the aqueous extract stimulated elongation of radicle and plumule as well as seedling biomass of both bean and corn, while the highest concentration (6.0%) was inhibitory. In addition, the growth of corn seedlings was retarded at the modest dose (4%) of the aqueous extract, while that for bean seedlings was promoted at the same concentration. Similarly, water soluble allelochemicals extracted from A. gaecorum shoots influenced accumulation of soluble sugars and proteins in a concentration and species dependent manner.
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Saleh, A. M., M. M. Y. Madany, and L. González, "The Effect of Coumarin Application on Early Growth and Some Physiological Parameters in Faba Bean (Vicia faba L.)", Journal of Plant Growth Regulation, vol. 34, pp. 233-241, 2015. AbstractWebsite

Many coumarins have been identified from natural sources, especially green plants. These compounds affect many plant activities and can also control growth processes. The effect of coumarin (COU) on germination, early growth, nutrient mobilization, and some physiologi- cal parameters of faba bean (Vicia faba L.) was researched. Seeds of faba bean were primed with different concentra- tions of COU (0.5, 1.0, 2.0, and 4.0 mM) to elucidate the effect on germination and nutrient mobilization. Accord- ingly, a greenhouse pot experiment was conducted to study the effect of 1.0 mM COU, as a seed priming treatment alone or in combination with foliar application, on the growth parameters, some biochemical constituents from primary and secondary metabolism and phytohormones of faba bean. The impact of COU was more pronounced on growth than germination, and was dependent on concen- tration and the mode of application. Both COU treatments significantly improved the level of primary and secondary metabolites as well as phytohormones. These data suggest that COU can affect the growth and physiology of faba bean either directly, as an active growth substance, or indirectly by its interaction with the metabolism of phytohormones.

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