Abdelgwad, M., D. Sabry, L. M. Abdelgawad*, and D. Mohamed Elroby Ali, "In Vitro Differential Sensitivity of Head and Neck Squamous Cell Carcinoma to Cisplatin, Silver Nanoparticles, and Photodynamic Therapy", Reports of Biochemistry & Molecular Biology , vol. 11, issue 2, pp. 224-237, 2022. abstract.pdf
Abdelgawad*, L. M., M. H. Nghnughi, and M. Abdelgwad, "Influence of photo biomodulation using 980 nm diode laser and exsosomes derived from dental pulp stem cells on pulp regeneration of dogs’ teeth ", Journal of medical pharmaceutical and allied sciences, , vol. 11, issue 3, pp. 4980 – 4985, 2022. abstract.pdf
Nassar, M. A. M., L. M. Abdelgawad, M. E. KHALLAF, D. H. E. L. ROUBY, D. Sabry, and M. M. RADWAN, "Synthesis, physical and mechanical properties of an experimental nano calcium aluminate/tri calcium silicate root repair material compared to mineral trioxide aggregate and Biodentine. (Part one)", Brazilian Dental Science, vol. 25, issue 4, pp. 1-10, 2022. abstract.pdf
Abdelgawad, L. M., N. A. A. ElShafei, S. A. Eissa, and D. Y. Ibrahim, " Efficacy of Photoinduced Photoacoustic Streaming and Diode Laser Irrigation Techniques on Smear Layer Removal, Sealer Penetration and Push-out Bond Strength", Journal of Lasers in Medical Sciences, vol. 13, pp. 1-10, 2022.
application/pdf iconefficacy_of_photoinduced_photoacoustic_streaming.pdf
AbdelatifAbdelaziz, A., A. M. Saafan, L. M. Abdelgawad, and M. B. E. badawy, "Phototherapy with Gold Nanoparticles and a Diode Laser for Oral Squamous Cell Carcinoma of the Tongue in Rats", Annals of R.S.C.B., vol. 25, issue 6, pp. 7570 - 7585, 2021.
M Abdelgawad, L., N. Salah, D. Sabry, and M. Abdelgwad, "Efficacy of Photobiomodulation and Vitamin D on Odontogenic Activity of Human Dental Pulp Stem Cells.", Journal of lasers in medical sciences, vol. 12, pp. e30, 2021. Abstract

The regeneration of dental pulp tissue using human dental pulp stem cells (HDPSCs) has attracted increasing attention in recent years. Recent studies have suggested that several factors such as photobiomodulation (PBM) and vitamin D affect the proliferation and differentiation of HDPSCs. Therefore, the present study evaluated the effects of PBM and vitamin D on odontogenic differentiation of HDPSCs for dentin -like tissue formation. HDPSCs were collected, isolated, and characterized and then divided into six groups: group I, control; group II, vitamin D (10 Mol); group III, irradiation at 1 J/cm of 810 nm diode laser; group IV, irradiation at 1 J/cm and culture with vitamin D; group V, irradiation at 2 J/cm, and group VI, irradiation at 2 J/cm and culture with vitamin D, cell viability assay was measured through MTT. Alkaline phosphatase (ALP) enzyme activity and mRNA levels of vascular endothelial growth factor (VEGF), bone morphogenic protein-2 (BMP-2), and dentin sialophosphoprotein (DSPP) were also assessed. PBM at 1 and 2 J/cm combined with vitamin D significantly promoted HDPSCs proliferation through MTT assay and odontogenic differentiation through gene expression of VEGF, BMP-2, and DSPP levels ( < 0.0001). PBM at 2 J/cm combined with vitamin D enhanced the HDPSCs proliferation and odontogenic differentiation and thus could be a novel strategy for dentin regeneration in dentistry.

Abdelgawad, L. M., M. M. Abd El-Hamed, D. Sabry, and M. Abdelgwad, "Efficacy of Photobiomodulation and Metformin on Diabetic Cell Line of Human Periodontal Ligament Stem Cells through Pathway.", Reports of biochemistry & molecular biology, vol. 10, issue 1, pp. 30-40, 2021. Abstract

BACKGROUND: Diabetes mellitus (DM) is a metabolic disorder resulting from hyperglycemia. Hyperglycemia contributes to oxidative stress, and the release of advanced glycation end products (AGEs) further promotes disease pathogenesis. Uncontrolled diabetes reflects great oral complications and affects human oral health. So, the present study aimed to assess the effects of photobiomodulation therapy (PBMT) and Metformin on proliferation and viability of human periodontal ligament stem cells (HPDLSCs) cultured in high glucose medium.

METHODS: HPDLSCs were collected, isolated, and characterized and then divided into eight groups. Addition of extra glucose to diabetic groups 24 hours before cell irradiations. Metformin was added to half of the diabetic groups. Cells were irradiated with 808 nm diode laser 24, 48 hours. Cell viability was analyzed with MTT assay 24 hours post-irradiation to detect cell viability in each group. Real-time (PCR) was used to evaluate gene expression of and and the effect of PBMT on Pathway. ELISA reader was used to evaluating cell viability through (ROS, TNF-α, IL-10) protein levels after cell irradiation.

RESULTS: Photobiomodulation at 1, 2, and 3 J/cm2 combined with metformin significantly promoted diabetic cell lines of HPDLSCs viability (in MTT assay and ELISA reader of ROS, TNF-α, IL-10 results) and gene expression of , and levels (p< 0.05).

CONCLUSION: photobiomodulation with 3 J/cm combined with metformin enhanced proliferation and viability of diabetic cell lines of HPDLSCs and thus could improve differentiation and function of diabetic cell lines of HPDLSCs with minimum side effects.