Hashim, S. M., E. Ismael, M. Tarek, F. F. Mohammed, F. A. A. reheem, and R. E. Doghaim, "Genetic Characterization and Pathological Evaluation of Clade 2.3.4.4b Avian Influenza Virus(H5N8) in Naturally Infected Domestic Ducks in Egyptian Farms", Adv. Anim. Vet. Sci., vol. 10, issue 12, pp. 2609-2621., 2022. 2022_-_genetic_characterization_and_pathological_evaluation_of_clade.pdf
Fayed, R. H., E. Rashad, S. I. El-Samannoudy, H. M. R. Elsherif, H. A. Ella, and E. Ismael, "Mitigative Effect of Dietary Tinospora cordifolia and Andrographis paniculata on Health and Hepato-renal Expression of Caspase-3 and TNF-α of Broiler Chickens Fed on Aflatoxin and Ochratoxin Contaminated Diet", Journal of Advanced Veterinary Research, vol. 13, no. 8, pp. 1668-1677, Oct., 2023. AbstractWebsite

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Hussien, A., E. Ismael, E. M. M. Elleithy, S. Kamel, D. A. Hamza, E. Y. Ismail, and K. N. E. -din Fahmy, "Influence of Bacillus subtilis and Bacillus licheniformis Probiotic Supplementation via the Drinking Water on Performance and Gut Health of Broiler Chickens", Journal of Advanced Veterinary Research, vol. 13, no. 1, pp. 94-102, Jan., 2023. AbstractWebsite

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Khalf, M. A. M., E. Ismael, and B. M. Bawish, "Comparing the Efficacy of Feed Withdrawal and Corn Diet Systems for Induced Molting on Health and Performance of Broiler Breeder Flocks", Journal of Advanced Veterinary Research, vol. 13, no. 8, pp. 1634-1641, Oct., 2023. AbstractWebsite

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Soliman, S. M., N. H. Aljahdali, K. A. Majrashi, S. M. El-Gameel, E. Ismael, H. M. Salem, M. A. Mahmoud, N. M. Albaqami, H. A. Mahjoub, M. T. El-Saadony, et al., "Immune Cell Reaction Associated with Coenurus cerebralis Infection in Sheep with Particular Reference to ELISA as a Diagnostic Tool", Life, vol. 12, pp. 1515, 2022.
Hussien, A., E. Ismael, B. M. Bawish, S. Kamel, E. Y. Ismail, E. K. El Bendari, and K. N. E. -din Fahmy, "Response of Broiler Chickens to the Dietary Fortification of Bile Acid", Journal of Advanced Veterinary Research, vol. 12, no. 5, pp. 582-587, Oct., 2022. AbstractWebsite

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Ismael, E., K. M. Fahim, S. M. O. Ghorab, R. H. Hamouda, A. M. Rady, M. M. Zaki, and A. M. Gamal, "Sustainable recycling of poultry eggshell waste for the synthesis of calcium oxide nanoparticles and evaluating its antibacterial potency against food-borne pathogens", Journal of Advanced Veterinary Research, vol. 14, no. 1, pp. 130 – 134, 2024. Abstract2024_-_sustainable_recycling_of_poultry_eggshell_waste.pdfWebsite

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Ismael, E., E. M. A. N. M. ISMAIL, H. A. N. A. N. S. A. A. D. KHALEFA, E. M. M. Elleithy, S. H. Elmosalamy, S. Marouf, and K. N. E. -din Fahmy, "Evaluation of Saccharomyces cerevisiae Yeast Fermentate and Xylanase in Reduced Energy Diet Fed to Broiler Chicken", International Journal of Veterinary Science, vol. 11, no. 2, pp. 141 – 150, 2022. Abstract2022_-_yeast_fermentate_and_xylanase.pdfWebsite

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Eissa, A. E., M. M. Attia, R. A. El Zlitne, A. A. Magdy, A. Edrees, M. S. Sharaf, A. E. Mahmoud, A. A. Abdelbaky, R. R. Abd ElMaged, E. Ismael, et al., The puzzling etiologies of transient black discoloration in Nile Tilapia (Oreochromis niloticus) intensively cultured under RAS system, , vol. 32, issue 1, pp. 581 - 592, 2024. Abstract2023_-_the_puzzling_etiologies_of_transient_black_discoloration_in_nile_tilapia.pdfWebsite

The aim of this study is to identify the hidden etiologies of the black chromo-shifting transient phenomenon affecting Nile tilapia, Oreochromis niloticus, farmed in RAS-controlled ponds together with the assessment of the immunological reaction against the chronic irritating effects of the invasive agent. A total of 100 Nile Tilapia were collected from a private farm at Kafrelsheikh Province, located on the northern side of the Egyptian Nile Delta. The clinical history of the affected fish farm showed an unknown chromo-shifting phenomenon where tilapias were exhibiting severe black skin coloration, which gradually disappeared after removal from the tank’s water. A comprehensive gross examination of the collected fish; including parasitological examination of skin and gill scraps, was performed. Blood biochemical testing was performed on the infested blackish O. niloticus and control non-infested fish. The current study showed that the monogenean parasite, Gyrodactylus cichlidarum, was the abundant parasite detected in the infested fish leading to abnormal black discoloration of skin and disruption of the immune system represented by significant increase of cortisol levels, lysozyme activity and different liver enzymes compared to the control. Treatment trials have been applied with moderate degrees of success, where the monogenean count was sharply decreased, and the normal skin color was remarkably restored, at the end of day 14 post-treatment. A triple treatment plan was initiated through 7 days’ application of 0.7 g/m3 copper sulfates pentahydrate preceded by 1.5 ml/m3 hydrogen peroxide 40% solution for the same period. One day after the end of the initial treatment, a maintenance dosage of 0.095 ml/m3 of glutaraldehyde (15%)/quaternary ammonium compounds (QACs) mixture was administered for 3 days. As a supportive/immune-stimulant regimen, a weekly dosage of vitamin C (0.45 g g/m3) and Saccharomyces cerevisiae (0.45 g/m3) was added into the tank’s water to improve the general fish health.

Abo-Sriea, T. M., E. Ismael, B. M. Sobhi, N. H. Hassan, S. A. E. Omar, A. M. Soliman, K. N. E. -din Fahmy, and A. Ramadan, "Impact of dietary-nucleotides and Saccharomyces cerevisiae-derivatives on growth-performance, antioxidant-capacity, immune-response, small-intestine histomorphometry, caecal-Clostridia, and litter-hygiene of broiler-chickens treated with florfenicol", International Journal of Veterinary Science and Medicine, vol. 12, no. 1: Taylor & Francis, pp. 11–24, 2024. Abstract2024_-_impact_of_dietary-nucleotides_and_saccharomyces_cerevisiae.pdfWebsite

Stress in poultry production is energy-demanding. Nucleotides and yeast cell-wall products are essential nutrients for broiler performance, gut function, and immune response. Antibiotics, like florfenicol, negatively affect the immune system. A total of 600 one-d-old broiler chickens (Cobb-500) were weighed and randomly allotted into four groups with three replicates each. The control group (G1) received the basal diet, G2 received a diet supplemented with a combination of nucleotides and Saccharomyces cerevisiae derivatives (250 g/Ton), G3 received the basal diet and medicated with florfenicol (25 mg/Kg body weight) in drinking water for 5 days, while G4 received a combination of nucleotides and Saccharomyces cerevisiae-derivatives (250 g/Ton) and medicated with florfenicol in drinking water. Growth performance criteria were recorded weekly. Blood, intestinal contents, small-intestine sections, and litter samples were collected to measure birds’ performance, carcass yields, leukocytic counts, antioxidant capacity, antibody titres, phagocytic index, caecal Clostridia, intestinal histomorphometry, and litter hygiene. Nucleotide-supplemented groups (G2 and G4) revealed significant (p ≤ 0.05) improvements in feed conversion, and body weight, but not for carcass yields in comparison to the control. Dietary nucleotides in G2 elevated blood total proteins, leucocytic count, antioxidant capacity, and phagocytic index, while they lowered blood lipids and litter moisture and nitrogen (p ≤ 0.05). Dietary nucleotides in G4 ameliorated the immunosuppressive effect of florfenicol (p ≤ 0.05) indicated in reducing caecal Clostridia, improving duodenal and ileal villi length, and increasing blood albumin and globulin levels, and phagocytosis%. Supplementing diets with nucleotides and yeast products has improved the immune system and provided a healthier gut for broilers.

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