, vol. 34, issue 6, pp. 485-493, 2022.
OBJECTIVE: To evaluate bioactivity and osteogenic potential of calcium silicate (CS)-doped iron oxide (FeO) nanoparticles versus pure CS in the reconstruction of induced critical-sized mandibular defects.
DESIGN: CS-doped FeO was prepared; morphological and microstructure identification of nanoparticles were made. An randomised design was developed on 24 adult male dogs where four critical-sized mandibular defects were created in each dog. Bone defects were allocated into control, CS, CS-3% FeO and CS-10% FeO group. Dogs were euthanized at 1 and 3 months (12 dog/time) for histopathologic and histomorphometric evaluation.
RESULTS: At three months, bone formation and maturation were evident where mean ± SD percent of mature bone was 2.66 ± 1.8, 9.9 ± 2.5, 22.9 ± 4.9, and 38.6 ± 8.1 in control, CS, CS-3% FeO, and CS-10% FeO groups respectively. A high significant ( < 0.001) increase in area percent of mature bone was recorded in CS, CS-3% FeO, and CS- 10% FeO groups compared to control group (73%, 88% and 93.3% respectively). Significant increase ( < 0.001) in area of mature bone was recorded in CS-3% FeO and CS-10% FeO groups compared to CS group. A significant increase ( < 0.001) in area of mature bone formation was detected in CS-10% FeO group compared to other groups.
CONCLUSION: CS-doped FeO has good osteoconductive, biocompatible properties with promoted bone regeneration. FeO has synergistic effect in combination with CS to promote bone formation. Increasing concentration of FeO nanoparticles resulted in improved osteogenesis and maturation. Results suggests that the novel CS-FeO alloplasts could be used for reconstruction of critical-sized bone defects.
