Other

Awards

  1. Awards of the Cairo University International publications (May 2011 and May 2012)
  2. Award of the best Ph.D.

Awards

  1. Awards of the Cairo University International publications (May 2011 and May 2012)
  2. Award of the best Ph.D.
Hilali, M., A. - A. Wahab, A. S. Z. -Maogood, and A. M. ElBehairy, "Comparative study between the epimastigote stage infecting Cephalopina titillator (Diptera: Oestridae) and Trypanosoma evansi infecting camels (Camelus dromedarius).", Egyptian Veterinary Medical Society of Parasitology Journal, vol. 6, pp. 1-11, 2010. Abstract

This study aimed to finding possible relationship between epimastigote stage infecting Cephalopina titillator and Trypanosoma evansi infecting camels in Egypt. Nine rats were assigned into three equal groups and used for experimental infection. Rats of group 1 were inoculated intraperitoneally with 0.5ml of camel blood infected with T. evansi. Group 2 was inoculated intraperitoneally with 0.5ml of the fluid content of 3rd stage larvae of C. titillator infected with epimastigote stage. The 3rd group was kept as non-infected control. Blood smears were obtained daily from each rat of the experimental and control rats. Rats in group 1 showed the parasites in their blood on the 2nd day post infection (DPI) until the end of experiment 1month post infection (P.I.). The rats of group 2 and 3 showed no trypomastigote in their blood for a period of 1 month post inoculation. Sera were obtained from 1st, 2nd and 3rd groups at weekly interval starting from 1st week for a period of 4 weeks. The sera were tested using test kit CATT/ T. evansi. The sera of rats in group1 showed positive results in CATT from the 1st to the 4th week post inoculation. The rats of group 2 were positive for CATT at 3rd week and 4th week PI. While rats in group3 were negative for CATT during the experiment. Genomic DNA from purified trypanosomes and the fluid of 3rd stage larvae of C. titillator was extracted and DNA subjected to PCR technique. Also, PCR products of the two samples were purified from gel and the insert was sequenced and analyzed. The DNA from epimastigote form of C. titillator and that of T. evansi were positive for T. evansi by PCR using specific primer at 227bp. The result of gene sequence showed that the region of gene 227bp was identical in T. evansi and epimastigote stage obtained from C. titillator.

ElBehairy, A. M., A. K. Ibrahim, W. S. Awad, A. Abdel-Gawad, M. M. Fahmy, and W. Mousa, "Efficacy of excretory/secretory and cathepsin L antigens for immunization of sheep against Fasciola gigantica infection.", The First International Conference for Application of Biotechnology, MSA Egypt and Greenwich University, England., 18-19 October, 2008.
Abdel-Gwad, A., M. M. Fahmy, W. Mousa, and A. ElBehairy, "Immuno-characterization of some Eimeria spp. infecting chicken in Egypt.", 21st International Conference WAAVP, Belgium, 19-23 August, 2007.
Shariati, F., J.L.Pérez-Arellano, A. L. Ruano, J. López-Abán, A. ElBehairy, B. Vicente, and A. Muro, "ANGIOGENESIS AND STRONGYLIODIASIS", XI Congresso Iberico de Parasitologia, Portuguese, Acta Parasitologica Portuguesa, pp. 97, 2009. Conference Proceeding Published Paper
Ibrahim, A. K., A. M. ElBehairy, M. K.A, and W. S. Awad, "Clinical and laboratory diagnosis of piroplasmids in naturally infected cattle in Egypt.", J.Egypt.vet.med.Assoc., vol. 69, issue 2, pp. 197-209, 2009. Abstract

The present study provides clinical and laboratory examination of 54 cattle for infection by Theileria and Babesia piroplasmids. Prevalence of the clinical signs revealed high percentage of the infected animals showed pale mucous membrane (42.59%) and fever (38.88%), while low percentage showed swelling of lymph nodes and hematuria (18.51%). PCR result discovered the majority of Babesia spp infection (26%), most of them infected by B. bigemina (17%). The percentages of positive animals for Theileria annulata were 13% of total 22% Theileria infection. The use of PCR resulted in significantly higher efficacy of detection of bovine piroplasmids compared to microscopical examination of blood smears and allowed the specific discrimination between pathogenic and non-pathogenic Theileria spp which cannot be accomplished by traditional diagnosis. ELISA revealed higher babesiosis and theileriosis infection percentage (39% and 28% respectively) than that of PCR. The hematological and biochemical changes are demonstrated in this study.

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