Musa, A., S. R. Ahmed, S. Hussein, K. A. Youssif, A. H. El-Ghorab, R. A. A. Haidari, M. A. H. Mostafa, M. Almaghrabi, T. Y. Aldakhil, M. A. Mohamed, et al., "Prominent antidiabetic and anticancer investigation of Scrophularia deserti extract: Integration of experimental and computational approaches", Journal of Molecular Structure, vol. 1315, issue -, pp. 1-16, 06/2024.
Musa, A., M. M. Al-Sanea, N. H. Alotaibi, T. S. Alnusaire, S. R. Ahmed, and E. M. Mostafa, "In silico study, protein kinase inhibition and antiproliferative potential of flavonoids isolated from Bassia eriophora (Schrad.) growing in KSA", Indian Journal of Pharmaceutical Education and Research, vol. 55, issue 2, pp. 483-490, 2021. article_no._4.pdf
Musa, A., N. H. Shady, S. R. Ahmed, T. S. Alnusaire, A. M. Sayed, B. F. Alowaiesh, I. Sabouni, M. M. Al-Sanea, E. M. Mostafa, K. A. Youssif, et al., "Antiulcer potential of olea europea l. Cv. arbequina leaf extract supported by metabolic profiling and molecular docking", Antioxidants, vol. 10, issue 5, pp. 644-660, 2021.
Hendawy, O., H. A. M. Gomaa, S. Hussein, S. I. Alzarea, S. Qasim, F. E. - Z. A. S. Rahman, A. T. Ali, and S. R. Ahmed, "Cold-pressed raspberry seeds oil ameliorates high-fat diet triggered non-alcoholic fatty liver disease", Saudi Pharmaceutical Journal, vol. 29, issue 11, pp. 1303-1313, 2021. article_no._5.pdf
El-Sherei, M. M., W. T. Islam, R. S. El-Dine, S. A. El-Toumy, and S. R. Ahmed, "Phytochemical investigation of the cytotoxic latex of Euphorbia cooperi N.E.Br.", Australian Journal of Basic and Applied Sciences, vol. 9, issue 11, pp. 488-493, 2015. Abstract

Background: Many investigations have been performed on the cytotoxic activity of different Euphorbia species and proved to possess moderate to strong cytotoxic effect on different human cancer cell lines. Objective: Current study aim is to determine the cytotoxic activity of the chloroform fraction derived from Euphorbia cooperi N. E. Br. latex methanolic extract on three human cancer cell lines, namely, breast cancer (MCF7), hepatocellular carcinoma (HepG2), and cervix cancer (HELA) cells in comparison to normal human melanocyte (HFB4) using Sulforhodamine B (SRB) assay. In addition, isolation and identification of the chemical constituents that might be responsible for the cytotoxic effect will be carried out. Results: The chloroform fraction showed potent cytotoxic activity against MCF7 cell line (IC50 = 4.23 ± 0.08 μg/ml), moderate activity against HepG2 (IC50 = 10.8 ± 0.74 μg/ml) and weak activity against HELA (IC50 = 26.6 ± 2.1 μg/ml) compared to standard doxorubicin (IC50 = 3.3 ± 0.1, 4.8 ± 0.14, 4.2 ± 0.3 μg/ml, respectively). The chloroform fraction indicated its possible selectivity against cancer cells rather than normal cells (IC50 = 15.6 ± 1.15 μg/ml on HFB4) compared to doxorubicin (IC50 = 4.0 ± 0.21 μg/ml on HFB4). A triterpene, euphol (1), one steroid, obtusifoliol (2) together with one diterpenoid with tigliane skeleton, 12-deoxyphorbol-13-isobutyrate-16-angelate-20-acetate (3) were isolated for the first time from the chloroform fraction of the latex of the plant under investigation. The structures of the isolated compounds were established on the basis of physical and spectroscopic analysis, including 1D and 2D NMR experiments and by comparison with the literature data. Conclusion: The present study demonstrated that the chloroform fraction of the latex of E. cooperi N. E. Br. showed promising cytotoxic activity especially against breast cancer.

Michel, C., M. El-sherei, W. Islam, A. Sleem, and S. Ahmed, Bioactivity-guided fractionation of the stem bark extract of Pterocarpus dalbergioides Roxb. ex Dc growing in Egypt , , vol. 51, issue 1, pp. 1-5, 2013. Abstract

A bioactivity-guided investigation of the ethanolic extract (70%) of the stem bark of Pterocarpus dalbergioides Roxb. ex DC revealed that the butanol fraction possessed potent antihyperglycemic and anti-inflammatory activities at a dose of 100 mg/kg b.wt. compared to metformin (150 mg/kg b.wt.) and indomethacin (20 mg/kg b.wt.) respectively. Two phenolic acids viz: gentisic (1) and gallic (3) acids and isoflavone genistin (2) were isolated for the first time from the studied plant adopting a bioactivity-guided fractionation. Identification of the isolated compounds was achieved using physical, chemical and spectroscopic data.