Khafagy, M. M., A. M. Mayla, M. H. Elberry, M. Gadalla, W. A. Gawad, and M. El-Dweik, "Postoperative anaerobic sepsis could be combated by prophylactic vaccination", Society for General Microbiology Annual Conference, Birmingham, UK, 30 March, 2015. Abstractabstract_of_birmingham.jpg

Background: Postoperative sepsis is the leading cause of morbidity and mortality after major surgery, resulting in hefty f inancial costs in hospitals all over the world.

Material and Methods: 40 Fischer rats were injected intra peritoneally with 0.05 ml Covexin 10 which contains toxoids from different clostridial species, 2 weeks prior to cecal ligation and puncture. Another 40 Fischer rats, as a control, underwent cecal ligation and puncture without vaccination.

Results: 16 of 40 vaccinated rats died (40%), peritoneal fluid cultures from the dead rats grew E. coli only, and 36 of 40 control rats died (90%), peritoneal fluid cultures from the dead rats were: 19 grew E. coli and C. perfringens; 12 grew E. coli, C. perfringens, and enterococci; 3 grew E. coli only; 1 grew E. coli, and enterocci; and 1 grew no organisms.

Conclusion: Prophylactic vaccination with clostridial toxoids prove effectiveness in preventing anaerobic infection, and reduce mortality in rats that underwent cecal ligation and puncture; the gold standard model for polymicrobial sepsis. Currently, a pilot study is underway in which human patients who will undergo major surgery are prophylactically vaccinated with clostridial toxoids, in an attempt to eradicate postoperative anaerobic sepsis.

Abdallah, Z. F., S. A. Loutfy, M. Shaalan, M. H. Elberry, A. M. Mayla, M. Moneer, and A. - R. N. Zekri, "Presence of Mouse Mammary Tumor Virus (MMTV) like env Sequences among Women with Familial and Non-Familial Breast Cancer", 5th International Conference of Virology , Hurgada, Egypt, 2014/12. Abstractesv.jpeg
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Viruses are considered to be one of the external high-risk factors closely related to human breast cancer. However, different studies of viruses in breast cancer present conflicting results and some of these works remain in dispute. The involvement of a retrovirus homologous to the mouse mammary tumor virus (MMTV) in the pathogenesis of human breast cancer (BC) has long been assumed, but has never been proven.

Therefore we aimed at investigating frequency of MMTV-like env sequences among familial and non-familial breast cancer clinical specimens obtained from Egyptian women at National Cancer Institute-Cairo University. Also presence of MMTV DNA infection and its association with some high-risk factors like old age, negative hormonal receptors, high grade of tumor, and large tumor size (> 5 cm) and their possible impact on outcome of breast cancer disease. Thirty cases breast cancer patients with a family history, and fifty cases (tumor and normal tissues) from the same breast cancer patients without a family history. All patients were subjected to PCR assay to determine the MMTV DNA positivity using specific primers targeted env gene.

Results showed that the number of non-familial breast cancer patients with positive ER was significantly higher than the corresponding number in familial breast cancer patients (84% vs. 63.3%, *p*=0.031) in non-familial and familial breast cancer respectively). Frequencies of MMTV-like env sequences among the patients' group using PCR assay were detected in a high proportion (79%, 70%) of Egyptian Women diagnosed with BC non-familial and familial group, respectively, while the control group recorded frequency of MMTV-like env sequences infection was (52%) that obtained from the same patient with sporadic or non-familial breast cancer. Also positivity of MMTV-DNA-like env sequences was significantly associated with tumor size (*p*=0.021) in non-familial group. We found that MMTV DNA-like env sequences was detected in 70% and 75% of familial and non-familial breast cancer in women.

Mayla, A. M., Human Associated Mycoplasmas, , Alexandria, Egypt, 2010. graduation_essay_2010.pdf
Khafagy, M. M., A. M. Mayla, A. Khafagy, M. Gadallah, A. Orabi, W. A. Gawad, N. Braya, G. Emira, and M. Eldweik, "Drop of post-operative infection after pre-operative vaccination with anaerobic toxoids (a clinical study of 445 patients)", Microbiology Society Annual Conference 2016, Liverpool, U.K., Microbiology Society, pp. 90, 2016. abstract_of_liverpool.jpg
Mayla, A. M., W. S. Mohamed, A. - R. N. Zekri, N. A. Gouda, M. M. Lotfy, M. G. Seadawy, M. A. - S. Elgohary, and Z. F. Abdallah, Co-infections and Reactivation of some Herpesviruses (HHV) and Measles Virus (MeV) in Egyptian Cancer Patients infected with Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), , vol. 37, issue 1, pp. 19, 2025. Abstract
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Coinfections and reactivation of persistent or latent viral infections such as herpesviruses (HHV) and/or measles virus (MeV) have been reported among COVID-19 patients. However, there is limited information regarding cancer patients who experienced severe acute respiratory syndrome corona virus-2 (SARS-CoV-2). The primary purpose of this study was to investigate the interplay between SARS-CoV-2, HHV and MeV in cancer patients, aiming to provide insights into the pathophysiology of these infections and to enhance the patients’ health outcomes.

El-Din, H. A. M., S. A. Loutfy, S. A. Kassem, A. M. Mayla, M. Elberry, N. Fathy, and A. Naqvi, "Virtual screening & molecular docking for discovering new small molecules against Ebola VP40 protein", 26th Annual Meeting of the Society for Virology, Münster, Germany, Society for Virology, pp. 181, 2016. abstract_of_munster.jpg
El-Din, H. A. M., S. A. Loutfy, N. Fathy, M. H. Elberry, A. M. Mayla, S. A. Kassem, and A. Naqvi, "Molecular docking based screening of compounds against VP40 from Ebola virus", Bioinformation, vol. 12, issue 3, pp. 192-196, 2016. molecular_docking_based_screening_of_compounds_against_vp40_from_ebola_virus.pdf
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