Hassan, K., J. King, M. El-Kady, M. Afifi, H. Abozeid, A. Pohlmann, M. Beer, and T. Harder, "Novel Reassortant Highly Pathogenic Avian Influenza A(H5N2) Virus in Broiler Chickens, Egypt", Emerging Infectious Disease journal, vol. 26, issue 1, pp. 129, 2020. AbstractWebsite

We detected a novel reassortant highly pathogenic avian influenza A(H5N2) virus in 3 poultry farms in Egypt. The virus carried genome segments of a pigeon H9N2 influenza virus detected in 2014, a nucleoprotein segment of contemporary chicken H9N2 viruses from Egypt, and hemagglutinin derived from the 2.3.4.4b H5N8 virus clade.

Awaad, M., M. Elmenawey, M. Afify, S. Zouelfekar, F. Mohammed, A. Samir, and V. Demy, The Impact of High Stocking Density and Saccharomyces cerevisiae boulardii on Productive Performance, Intestinal Microbiota and Gut Integrity of Broiler Chickens, , 2020/01/05, 2019. Abstracthigh_stocking_density_and_saccharomyces_cerevisiae_boulardii_210785.pdf

AB S T RA C T Day-old male Arbor Acres plus broiler chickens (n=1120) were used to study the effect of dietary supplementation of the Probiotic S. c. boulardii on production performance, intestinal microbiota and gut integrity under HSD challenge. Duration of the trial extended from one day of age up to slaughter (42 days). The birds were allotted into 4 groups (1-4). Those of groups 1(NC) and 3 (T1) consisted of 240 birds. While those of groups 2 (PC) and 4(T2) consisted of 320 birds each. All groups ran contemporaneously. The birds of each group assigned into 10 replicates. Those of groups NC and T1 consisted of 10 birds/replicate/m 2 floor area; while those of groups PC and T2 consisted of 13 birds/replicate/m 2 floor area. Chickens of groups T1 and T2 were dietary supplemented with S.c. bululardii; while PC and NC groups were kept as controls. All experimented birds were vaccinated against different diseases according to the vaccination programs usually adopted in Egyptian chicken broiler farms. The used dietary supplement in challenged and unchallenged HSD improved productive performance variables including BW, BWG, FCR. It improved V/C ratio, fecal and cecal LAB counts and GIT integrity. It also Reduced macroscopic and microscopic lesion scores post vVND virus challenge.

Abozeid, H. H., A. Paldurai, B. P. Varghese, S. K. Khattar, M. A. Afifi, S. Zouelfakkar, A. H. El-Deeb, M. F. El-Kady, and S. K. Samal, Development of a recombinant Newcastle disease virus-vectored vaccine for infectious bronchitis virus variant strains circulating in Egypt, , vol. 50, issue 1, pp. 12, 2019. AbstractWebsite

Infectious bronchitis virus (IBV) causes a major disease problem for the poultry industry worldwide. The currently used live-attenuated vaccines have the tendency to mutate and/or recombine with circulating field strains resulting in the emergence of vaccine-derived variant viruses. In order to circumvent these issues, and to develop a vaccine that is more relevant to Egypt and its neighboring countries, a recombinant avirulent Newcastle disease virus (rNDV) strain LaSota was constructed to express the codon-optimized S glycoprotein of the Egyptian IBV variant strain IBV/Ck/EG/CU/4/2014 belonging to GI-23 lineage, that is prevalent in Egypt and in the Middle East. A wild type and two modified versions of the IBV S protein were expressed individually by rNDV. A high level of S protein expression was detected in vitro by Western blot and immunofluorescence analyses. All rNDV-vectored IBV vaccine candidates were genetically stable, slightly attenuated and showed growth patterns comparable to that of parental rLaSota virus. Single-dose vaccination of 1-day-old SPF White Leghorn chicks with the rNDVs expressing IBV S protein provided significant protection against clinical disease after IBV challenge but did not show reduction in tracheal viral shedding. Single-dose vaccination also provided complete protection against virulent NDV challenge. However, prime-boost vaccination using rNDV expressing the wild type IBV S protein provided better protection, after IBV challenge, against clinical signs and significantly reduced tracheal viral shedding. These results indicate that the NDV-vectored IBV vaccines are promising bivalent vaccine candidates to control both infectious bronchitis and Newcastle disease in Egypt.

Abozeid, H., S. K. Khattar, A. H. El-Deeb, S. Zouelfakkar2, M. F. El-Kady, and S. K. Samal1, "Development of recombinant Newcastle disease virus-based vaccine expressing S protein of the Egyptian infectious bronchitis virus Ck/EG/CU/4/2014", American Society for Virology 37th Annual Meeting, University of Maryland, College Park, 16 July, 2018. IB recombinant variant vaccine on Lasota in Maryland
El-Kady, M. F., K. E. Hassan, M. A. Afifi, S. A. S. Shany, and A. Ali, "Extensive outbreaks of highly pathogenic avian influenza in Egypt: circulation of viruses belonging to two different clades in domestic chickens and ducks.", American Society of Virology 2018 Annual Meeting, held at the University of Maryland, College Park, July 14-18, 2018, 16 July, 2018. paper_1_american_society_for_virology.docx
Awaad, M. H. H., B. SHALABY, M. A. Aly, and S. Z. 1; E. - D. 2; F. A. M. F. and Mohammed, "Effect of Lactobacillus Acidophilus, Lactobacillus Acidophilus Plus Pichia Anomola and Lactobacillus Acidophilus Plus Pichia Anomola Plus Bacteriophage on Immune Responsiveness, Intestinal Enumeration of Clostridium Perfringens, Salmonella Typhimurium, Co", IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS), vol. Volume 7, , issue Issue 7 Ver. I, pp. PP 59-65, 2015. iosr_journal_of_agriculture_and_veterinary_science.docx
Manal, A. A., M. O. El Shazly, Z. A. Sahar, and A. M. H. H. and Iman, B. Shaheed., "Immunomodulatory properties of inactivated propiobacterium granulosum (IM-104 ) versus Vit E in cyclophosphamide immunosuppressed chickens.", XII International Congress World Vet. Poult. Assoc. Oral (80).pp. 244. 17-21 Sep., Egypt, sep 2001.
Hassan, M. K., A. A. Manal, and A. M. Mona, "Susceptibility of vaccinated and unvaccinated Egyptian chickens to very virulent infectious bursal disease virus. ", Avian Pathology, 31., vol. 31, pp. ,149-156, 2002.
Hassan, M. K., M. A. Afify, and M. M. Aly, "Genetic resistance of Egyptian chickens to infectious bursal disease and Newcastle disease.", Tropical Animal Health and Production, 36. 1-9., vol. 36, pp. 1-9, 2004.
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