Comparative study of the osteogenic potential of mesenchymal stem cells derived from different sources., Aboushady, Iman M., Salem Zeinab A., Sabry Dina, and Mohamed Abbas , Journal of clinical and experimental dentistry, 2018 Jan, Volume 10, Issue 1, p.e7-e13, (2018) Abstract

Background: Mesenchymal stem cells (MSCs) can regenerate missing tissues and treat diseases. Hence, the current work aimed to compare the proliferation rate and the osteogenic differentiation potential of bone marrow MSCs (BMSCs), gingival MSCs (GMSCs) and submandibular MSCs (SMSCs).

Material and Methods: MSCs derived from bone marrow, gingiva and submandibular salivary gland were isolated and cultured from rats. The proliferation capacity was judged by MTT proliferation Assay. Osteogenic differentiation was assessed by Alzarin red stain and quantitative RT-PCR was performed for Runx-2 and MMP-13.

Results: The highest significant proliferation was estimated in the BMSCs compared to GMSCs and SMSCs (-value was < 0.01). All studied cell types formed mineralized nodules as stained with Alizarin Red stain at the 3rd passage of differentiation. However, BMSCs seemed to generate the highest level of mineralization compared to GMSCs and SMSCs. RT-PCR revealed that the expression of Runx-2 and MMP-13 mRNAs was significantly increased in the BMSCs compared to GMSCs and SMSCs (-value was < 0.01).

Conclusions: BMSCs displayed maximum osteogenesis results followed by the GMSCs and lastly by the SGSCs. Thus, it could be recommended that GMSCs can be used as a second choice after BMSCs when bone tissue reconstruction is needed. Mesenchymal stem cells, osteogenic differentiation, Runx-2, MMP-13.

The Effect of Transplanted Bone Marrow Stem Cells on the Tongue of Irradiated Rats (Histological and Immunohistochemical study), Aboushady, I. M., Mubarak R. T., El-mougy S. A. F., Rashed L. A. 3, and El-desouky A. A. 4 , Journal of American Science, Volume 8, Issue 11, p.553-561, (2012) Abstract

Abstract: Normal tissue damage following radiotherapy (RT) is still a major problem in cancer treatment. So, the current work aimed at exploring the possible role of locally injected bone marrow derived mesenchymal stem cells (BM-MSCs) in ameliorating the side effects of ionizing radiation on the normal rat's tongue. Materials & Methods: Ten rats were used for isolating BM-MSCs, ten rats served as control group (G1) and twenty rats received a single radiation dose of 10 Gy to the head and neck region, then, they were equally divided into 2 experimental groups: Irradiated only group (G2) and Irradiated+MSCs group (G3). All animals were sacrificed at 2 weeks following irradiation. The tongue was examined histologically and immunohistochemically using anti-PCNA primary antibody. Results: Histological & immunohistochemical examination of the treated group (G3) revealed an obvious improvement in the histological structure of the tongue; compared to the irradiated group (G2); in addition to up regulated expression of PCNA, indicating improved cell proliferation rate. Conclusions: BM-MSCs have shown positive effect in protection against the side effects of radiotherapy on normal tissues, which was emphasized by their enhancing effect on the proliferative capacity of these tissues.