Dessouki, S. M., D. A. - E. R. Ahmed, and A. K. Fayed, "Sperm kinetics of Egyptian buffalo bulls () affected by the red laser postfreezing.", Journal of advanced veterinary and animal research, vol. 9, issue 3, pp. 396-404, 2022. Abstract

OBJECTIVE: To improve the fertilizing ability of frozen buffalo semen using the more beneficial, accurate, and cheap technique of laser irradiation at specific wavelengths and exposure times.

MATERIALS AND METHODS: The red laser source (625 nm) was used in this study with 5 watts output power and for the irradiation of the semen samples for 5 min; the laser focus spot area was 1 cm.Thirty straws belonging to five buffalo bulls were used in this study.

RESULTS: The results show that total motility (%) and progressive motility (%) increased insignificantly after 5 min of exposure (73.8 ± 1.4 and 60.4 ± 1.1, respectively) compared to the control sample (70.9 ± 0.9 and 57.5 ± 1.7, respectively). All velocity parameters (velocity average path,velocity curved line, and velocity straight line µm/sec) recorded a significant (p < 0.05) increase in samples measured 5 min after exposure (52.3 ± 1.3, 83.5 ± 2.0, and 43.5 ± 1.2, respectively) compared to the untreated ones (47.1 ± 2.0, 76.3 ± 3.1, and 38.6 ± 1.9, respectively).

CONCLUSION: The application of the red laser light on buffalo semen postthawing resulted in a positive correlation with almost every motility parameter; it may be recommended to apply this technique pre-in vitro fertilization for embryo production of buffalo species.

Dessouki, S. M., D. A. - E. R. Ahmed, and A. K. Fayed, "Sperm kinetics of Egyptian buffalo bulls () affected by the red laser postfreezing.", Journal of advanced veterinary and animal research, vol. 9, issue 3, pp. 396-404, 2022. Abstract

OBJECTIVE: To improve the fertilizing ability of frozen buffalo semen using the more beneficial, accurate, and cheap technique of laser irradiation at specific wavelengths and exposure times.

MATERIALS AND METHODS: The red laser source (625 nm) was used in this study with 5 watts output power and for the irradiation of the semen samples for 5 min; the laser focus spot area was 1 cm.Thirty straws belonging to five buffalo bulls were used in this study.

RESULTS: The results show that total motility (%) and progressive motility (%) increased insignificantly after 5 min of exposure (73.8 ± 1.4 and 60.4 ± 1.1, respectively) compared to the control sample (70.9 ± 0.9 and 57.5 ± 1.7, respectively). All velocity parameters (velocity average path,velocity curved line, and velocity straight line µm/sec) recorded a significant (p < 0.05) increase in samples measured 5 min after exposure (52.3 ± 1.3, 83.5 ± 2.0, and 43.5 ± 1.2, respectively) compared to the untreated ones (47.1 ± 2.0, 76.3 ± 3.1, and 38.6 ± 1.9, respectively).

CONCLUSION: The application of the red laser light on buffalo semen postthawing resulted in a positive correlation with almost every motility parameter; it may be recommended to apply this technique pre-in vitro fertilization for embryo production of buffalo species.

Fayed, A. K., A. El-Sayed, N. A. Ashmawy, and G. Ashour, "Blood metabolites and hormones levels as indicators for growth performance in Egyptian buffalo calves.", 2nd International Conference On Biotechnology Applications In Agriculture (ICBAA), Moshtohor and Hurghada: Benha University, pp. 17-24, 2014. Abstract
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